Hyperbaric oxygen therapy and coenzyme Q10 synergistically attenuates damage progression in spinal cord injury in a rat model

IF 2.7 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of chemical neuroanatomy Pub Date : 2023-10-01 DOI:10.1016/j.jchemneu.2023.102322

Alireza Ghaemi , Mohammad Ghiasvand , Melody Omraninava , Mohammed Yousif Merza , Adnan Taan Alkhafaji , Amir Raoofi , Davood Nasiry , Mohammad Darvishi , Reza Akhavan-Sigari

{"title":"Hyperbaric oxygen therapy and coenzyme Q10 synergistically attenuates damage progression in spinal cord injury in a rat model","authors":"Alireza Ghaemi , Mohammad Ghiasvand , Melody Omraninava , Mohammed Yousif Merza , Adnan Taan Alkhafaji , Amir Raoofi , Davood Nasiry , Mohammad Darvishi , Reza Akhavan-Sigari","doi":"10.1016/j.jchemneu.2023.102322","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><span>Identifying effective spinal cord injury (SCI) treatments remains a major challenge, and current approaches are still unable to effectively improve its. Currently, we investigated the combined effects of hyperbaric oxygen (HBO) along with </span>coenzyme Q10 (CoQ10) in the recovery of SCI in rats.</p></div><div><h3>Material and methods</h3><p>Ninety female mature Sprague-Dawley rats were allocated into five equal groups, including; sham group, SCI group, HBO group (underwent SCI and received HBO), CoQ10 group (underwent SCI and received CoQ10), and HBO+CoQ10 group (underwent SCI and received HBO plus CoQ10). Tissue samples at the lesion site were obtained for evaluation of stereological, immunohistochemical, biochemical, molecular. Also, functional tests were performed to evaluate of behavioral properties.</p></div><div><h3>Results</h3><p>We found that a significant increase in stereological parameters, biochemical factors (GSH, SOD and CAT), IL-10 gene expression and behavioral functions (BBB and EMG Latency) in the treatment groups, especially HBO+CoQ10 group, compared to SCI group. In addition, MDA levels, the density of apoptotic cells, as well as expression of inflammatory genes (TNF-α and IL-1β) were considerably reduced in the treatment groups, especially HBO+CoQ10 group, compared to SCI group.</p></div><div><h3>Conclusion</h3><p>We conclude that co-administration of HBO and HBO+CoQ10 has a synergistic neuroprotective effects in animals undergoing SCI.</p></div>","PeriodicalId":15324,"journal":{"name":"Journal of chemical neuroanatomy","volume":"132 ","pages":"Article 102322"},"PeriodicalIF":2.7000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chemical neuroanatomy","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0891061823000923","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 2

Abstract

Background

Identifying effective spinal cord injury (SCI) treatments remains a major challenge, and current approaches are still unable to effectively improve its. Currently, we investigated the combined effects of hyperbaric oxygen (HBO) along with coenzyme Q10 (CoQ10) in the recovery of SCI in rats.

Material and methods

Ninety female mature Sprague-Dawley rats were allocated into five equal groups, including; sham group, SCI group, HBO group (underwent SCI and received HBO), CoQ10 group (underwent SCI and received CoQ10), and HBO+CoQ10 group (underwent SCI and received HBO plus CoQ10). Tissue samples at the lesion site were obtained for evaluation of stereological, immunohistochemical, biochemical, molecular. Also, functional tests were performed to evaluate of behavioral properties.

Results

We found that a significant increase in stereological parameters, biochemical factors (GSH, SOD and CAT), IL-10 gene expression and behavioral functions (BBB and EMG Latency) in the treatment groups, especially HBO+CoQ10 group, compared to SCI group. In addition, MDA levels, the density of apoptotic cells, as well as expression of inflammatory genes (TNF-α and IL-1β) were considerably reduced in the treatment groups, especially HBO+CoQ10 group, compared to SCI group.

Conclusion

We conclude that co-administration of HBO and HBO+CoQ10 has a synergistic neuroprotective effects in animals undergoing SCI.

查看原文本刊更多论文

高压氧治疗和辅酶Q10协同减缓大鼠脊髓损伤模型的损伤进展

背景确定有效的脊髓损伤（SCI）治疗方法仍然是一个重大挑战，目前的方法仍然无法有效地改善它。目前，我们研究了高压氧（HBO）和辅酶Q10（CoQ10）在大鼠SCI恢复中的联合作用。材料与方法将90只雌性成年Sprague-Dawley大鼠随机分为5组，包括：；假手术组、SCI组、HBO组（接受SCI并接受HBO）、辅酶Q10组（接受SCI并接受辅酶Q10）和HBO+CoQ10组。获取病变部位的组织样本进行体视学、免疫组织化学、生物化学和分子生物学评估。此外，还进行了功能测试来评估行为特性。结果与SCI组相比，治疗组（尤其是HBO+CoQ10组）的体视学参数、生化因子（GSH、SOD和CAT）、IL-10基因表达和行为功能（BBB和EMG潜伏期）显著增加。此外，与SCI组相比，治疗组，尤其是HBO+CoQ10组的MDA水平、凋亡细胞密度以及炎症基因（TNF-α和IL-1β）的表达显著降低。结论HBO和HBO+CoQ10联合给药对SCI动物具有协同神经保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of chemical neuroanatomy 医学-神经科学

CiteScore

4.50

自引率

3.60%

发文量

审稿时长

62 days

期刊介绍： The Journal of Chemical Neuroanatomy publishes scientific reports relating the functional and biochemical aspects of the nervous system with its microanatomical organization. The scope of the journal concentrates on reports which combine microanatomical, biochemical, pharmacological and behavioural approaches. Papers should offer original data correlating the morphology of the nervous system (the brain and spinal cord in particular) with its biochemistry. The Journal of Chemical Neuroanatomy is particularly interested in publishing important studies performed with up-to-date methodology utilizing sensitive chemical microassays, hybridoma technology, immunocytochemistry, in situ hybridization and receptor radioautography, to name a few examples. The Journal of Chemical Neuroanatomy is the natural vehicle for integrated studies utilizing these approaches. The articles will be selected by the editorial board and invited reviewers on the basis of their excellence and potential contribution to this field of neurosciences. Both in vivo and in vitro integrated studies in chemical neuroanatomy are appropriate subjects of interest to the journal. These studies should relate only to vertebrate species with particular emphasis on the mammalian and primate nervous systems.