Chi K Hoang, Cuong H Le, Dat T Nguyen, Hang T N Tran, Chinh V Luu, Huong M Le, Ha T H Tran
{"title":"Steroid Components of Marine-Derived Fungal Strain <i>Penicillium levitum</i> N33.2 and Their Biological Activities.","authors":"Chi K Hoang, Cuong H Le, Dat T Nguyen, Hang T N Tran, Chinh V Luu, Huong M Le, Ha T H Tran","doi":"10.1080/12298093.2023.2248717","DOIUrl":null,"url":null,"abstract":"<p><p>Genus <i>Penicillium</i> comprising the most important and extensively studied fungi has been well-known as a rich source of secondary metabolites. Our study aimed to analyze and investigate biological activities, including <i>in vitro</i> anti-cancer, anti-inflammatory and anti-diabetic properties, of metabolites from a marine-derived fungus belonging to <i>P. levitum</i>. The chemical compounds in the culture broth of <i>P. levitum</i> strain N33.2 were extracted with ethyl acetate. Followingly, chemical analysis of the extract leaded to the isolation of three ergostane-type steroid components, namely cerevisterol <b>(1)</b>, ergosterol peroxide <b>(2)</b>, and (3β,5α,22E)-ergosta-6,8(14),22-triene-3,5-diol <b>(3)</b>. Among these, <b>(3)</b> was the most potent cytotoxic against human cancer cell lines Hep-G2, A549 and MCF-7 with IC<sub>50</sub> values of 2.89, 18.51, and 16.47 µg/mL, respectively, while the compound <b>(1)</b> showed no significant effect against tested cancer cells. Anti-inflammatory properties of purified compounds were evaluated based on NO-production in LPS-induced murine RAW264.7 macrophages. As a result, tested compounds performed diverse inhibitory effects on NO production by the macrophages, with the most significant inhibition rate of 81.37 ± 1.35% at 25 µg/mL by the compound <b>(2)</b>. Interestingly, compounds <b>(2)</b> and <b>(3)</b> exhibited inhibitory activities against pancreatic lipase and α-glucosidase enzymes <i>in vitro</i> assays. Our study brought out new data concerning the chemical properties and biological activities of isolated steroids from a <i>P. levitum</i> fungus.</p>","PeriodicalId":18825,"journal":{"name":"Mycobiology","volume":"51 4","pages":"246-255"},"PeriodicalIF":1.6000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/20/0e/TMYB_51_2248717.PMC10498798.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycobiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/12298093.2023.2248717","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
Genus Penicillium comprising the most important and extensively studied fungi has been well-known as a rich source of secondary metabolites. Our study aimed to analyze and investigate biological activities, including in vitro anti-cancer, anti-inflammatory and anti-diabetic properties, of metabolites from a marine-derived fungus belonging to P. levitum. The chemical compounds in the culture broth of P. levitum strain N33.2 were extracted with ethyl acetate. Followingly, chemical analysis of the extract leaded to the isolation of three ergostane-type steroid components, namely cerevisterol (1), ergosterol peroxide (2), and (3β,5α,22E)-ergosta-6,8(14),22-triene-3,5-diol (3). Among these, (3) was the most potent cytotoxic against human cancer cell lines Hep-G2, A549 and MCF-7 with IC50 values of 2.89, 18.51, and 16.47 µg/mL, respectively, while the compound (1) showed no significant effect against tested cancer cells. Anti-inflammatory properties of purified compounds were evaluated based on NO-production in LPS-induced murine RAW264.7 macrophages. As a result, tested compounds performed diverse inhibitory effects on NO production by the macrophages, with the most significant inhibition rate of 81.37 ± 1.35% at 25 µg/mL by the compound (2). Interestingly, compounds (2) and (3) exhibited inhibitory activities against pancreatic lipase and α-glucosidase enzymes in vitro assays. Our study brought out new data concerning the chemical properties and biological activities of isolated steroids from a P. levitum fungus.
期刊介绍:
Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.