Mate calling alters expression of neuropeptide, cocaine- and amphetamine- regulated transcript (CART) in the brain of male frog Microhyla nilphamariensis

IF 2.5 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

Neuropeptides Pub Date : 2023-09-09 DOI:10.1016/j.npep.2023.102380

Shobha Bhargava , Ketaki Shetye , Swapnil Shewale , Nitin Sawant , Sneha Sagarkar , Nishikant Subhedar

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引用次数: 0

Abstract

Croaking is a unique component of reproductive behaviour in amphibians which plays a key role in intraspecies communication and mate evaluation. While gonadal hormones are known to induce croaking, central regulation of sound production is less studied. Croaking is a dramatic, transient activity that sets apart an animal from non-croaking individuals. Herein, we aim at examining the profile of the neuropeptide cocaine- and amphetamine-regulated transcript (CART) in actively croaking and non-croaking frog Microhyla nilphamariensis. In anurans, this peptide is widely expressed in the areas inclusive of acoustical nuclei as well as areas relevant to reproduction. CART immunoreactivity was far more in the preoptic area (POA), anteroventral tegmentum (AV), ventral hypothalamus (vHy), pineal (P) and pituitary gland of croaking frog compared to non-croaking animals. On similar lines, tissue fragments collected from the mid region of the brain inclusive of POA, vHy, AV, pineal and pituitary gland of croaking frog showed upregulation of CART mRNA. However, CART immunoreactivity in the neuronal perikarya of raphe (Ra) was completely abolished during croaking activity. The data suggest that CART signaling in the brain may be an important player in mediating croaking behaviour in the frog.

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交配呼叫改变雄性尼法马里小蛙大脑中神经肽、可卡因和安非他命调节转录物(CART)的表达

交配是两栖动物繁殖行为的一个独特组成部分，在种内交流和配偶评估中起着关键作用。虽然已知性腺激素会引起沙哑，但对声音产生的中央调节研究较少。鸣叫是一种戏剧性的、短暂的活动，它将动物与不鸣叫的个体区分开来。在此，我们的目的是检测神经肽可卡因和苯丙胺调节转录物（CART）在活动性和非活动性尼罗滨蛙中的分布。在无核细胞中，这种肽在包括声核在内的区域以及与生殖相关的区域广泛表达。与非鸣叫动物相比，鸣叫蛙视前区（POA）、前腹侧被盖（AV）、腹侧下丘脑（vHy）、松果体（P）和垂体的CART免疫反应性更强。在类似的细胞系上，从包括POA、vHy、AV、松果体和脑垂体在内的脑中部收集的组织片段显示CART mRNA上调。然而，中缝（Ra）神经元周的CART免疫反应在鸣叫活动中完全消失。数据表明，大脑中的CART信号可能是介导青蛙鸣叫行为的重要因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuropeptides 医学-内分泌学与代谢

CiteScore

5.40

自引率

6.90%

发文量

审稿时长

>12 weeks

期刊介绍： The aim of Neuropeptides is the rapid publication of original research and review articles, dealing with the structure, distribution, actions and functions of peptides in the central and peripheral nervous systems. The explosion of research activity in this field has led to the identification of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems. The journal provides a unique opportunity of integrating the many disciplines involved in all neuropeptide research. The journal publishes articles on all aspects of the neuropeptide field, with particular emphasis on gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.