Acute Response of Engineered Cardiac Tissue to Pressure and Stretch.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2023-01-01 Epub Date: 2022-05-31 DOI:10.1159/000525250
Leslie Donoghue, Caleb Graham, Palaniappan Sethu
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引用次数: 0

Abstract

The heart is a dynamic organ, and the cardiac tissue experiences changes in pressure and stretch during the cardiac cycle. Existing cell culture and animal models are limited in their capacity to decouple and tune specific hemodynamic stresses implicated in the development of physiological and pathophysiological cardiac tissue remodeling. This study focused on creating a system to subject engineered cardiac tissue to either pressure or stretch stimuli in isolation and the subsequent evaluation of acute tissue remodeling. We developed a cardiac tissue chip containing three-dimensional (3-D) cell-laden hydrogel constructs and cultured them within systems where we could expose them to either pressure changes or volume changes as seen in the left ventricle. Acute cellular remodeling with each condition was qualitatively and quantitatively assessed using histology, immunohistochemistry, gene expression studies, and soluble factor analysis. Using our unique model system, we isolated the effects of pressure and stretch on engineered cardiac tissue. Our results confirm that both pressure and stretch mediate acute stress responses in the engineered cardiac tissue. However, both experimental conditions elicited a similar acute phase injury response within this timeframe. This study demonstrates our ability to subject engineered cardiac tissue to either pressure or stretch stimuli in isolation, both of which elicited acute tissue remodeling responses.

人造心脏组织对压力和拉伸的急性反应
心脏是一个动态器官,心脏组织在心动周期中会经历压力和伸展的变化。现有的细胞培养和动物模型在解耦和调节与心脏组织生理和病理重塑发展有关的特定血液动力学压力方面能力有限。本研究的重点是创建一个系统,使工程心脏组织单独承受压力或拉伸刺激,并随后评估急性组织重塑。我们开发了一种心脏组织芯片,其中包含三维(3-D)细胞负载水凝胶构建物,并将其培养在系统中,使其暴露于左心室中的压力变化或容积变化。我们使用组织学、免疫组织化学、基因表达研究和可溶性因子分析对每种条件下的急性细胞重塑进行了定性和定量评估。利用我们独特的模型系统,我们分离了压力和拉伸对工程心脏组织的影响。我们的结果证实,压力和拉伸都能介导工程心脏组织的急性应激反应。然而,两种实验条件在这一时间范围内都引起了类似的急性期损伤反应。这项研究表明,我们有能力让工程心脏组织单独接受压力或拉伸刺激,这两种刺激都会引起急性组织重塑反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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