Decontamination Validation of a Class II Type A2 Biosafety Cabinet during Laboratory Fumigation.

IF 0.5 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Applied Biosafety Pub Date : 2020-03-01 DOI:10.1177/1535676019890975

Greg Frey, Cathy Robertson, Jay Krishnan

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引用次数: 6

Abstract

Objective: The objective of this study was to evaluate whether a Class II type A2 biosafety cabinet in a laboratory could be decontaminated while the laboratory was being fumigated using vaporous hydrogen peroxide or peracetic acid dry fogging.

Methods: To validate decontamination of all parts of the biosafety cabinet, biological indicators were placed at various locations within the biosafety cabinet, including between the pleats of supply and exhaust HEPA filters. To assess whether the operational status of the biosafety cabinet influenced the outcome of its decontamination, fumigation validations were undertaken with the cabinet running and not running. The amount of fumigant and the duration of fumigation remained constant whether the biosafety cabinet was running or not.

Discussion: Biosafety cabinet decontamination was successful only when the cabinet was running to facilitate the fumigant's circulation within the plenums and across the HEPA filters. This study shows both vaporous hydrogen peroxide and peracetic acid dry fogging can be used successfully to decontaminate Class II type A2 biosafety cabinets during laboratory fumigation, provided the biosafety cabinets are operational and running during the fumigation.

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ⅱ类A2型生物安全柜在实验室熏蒸过程中的去污验证。

目的:本研究的目的是评估在实验室使用蒸汽过氧化氢或过氧乙酸干雾熏蒸时，是否可以对实验室II类A2型生物安全柜进行消毒。方法:为了验证生物安全柜各部位的去污效果，在生物安全柜内的各个位置，包括供气和排气HEPA过滤器褶之间放置生物指示剂。为了评估生物安全柜的运行状态是否影响其去污结果，在柜子运行和不运行的情况下进行了熏蒸验证。无论生物安全柜是否运行，熏蒸剂的用量和熏蒸时间都保持不变。讨论:只有当生物安全柜运行以促进熏蒸剂在全体会议和HEPA过滤器内的循环时，生物安全柜去污才能成功。本研究表明，在实验室熏蒸过程中，如果生物安全柜处于运行状态，则蒸汽过氧化氢和过氧乙酸干雾法都可以成功地用于II类A2型生物安全柜的去污。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Biosafety Environmental Science-Management, Monitoring, Policy and Law

CiteScore

2.50

自引率

13.30%

发文量

期刊介绍： Applied Biosafety (APB), sponsored by ABSA International, is a peer-reviewed, scientific journal committed to promoting global biosafety awareness and best practices to prevent occupational exposures and adverse environmental impacts related to biohazardous releases. APB provides a forum for exchanging sound biosafety and biosecurity initiatives by publishing original articles, review articles, letters to the editors, commentaries, and brief reviews. APB informs scientists, safety professionals, policymakers, engineers, architects, and governmental organizations. The journal is committed to publishing on topics significant in well-resourced countries as well as information relevant to underserved regions, engaging and cultivating the development of biosafety professionals globally.