The association between the level of specific IgE to molecular components of mites and the expression of CD23 molecule on B lymphocytes in atopic dermatitis patients treated or not treated with dupilumab—Pilot study

IF 4.6 2区医学 Q2 ALLERGY

Clinical and Translational Allergy Pub Date : 2023-07-22 DOI:10.1002/clt2.12278

Jarmila Čelakovská, Eva Čermáková, Petra Boudková, Ctirad Andrýs, Jan Krejsek

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引用次数: 0

Abstract

Background

The CD23 molecule has an effect on the regulation of IgE synthesis, either by stimulation or inhibition. It is not yet known whether the expression of CD23 on B lymphocytes is related to the level of allergen-specific IgE antibodies in patients with atopic dermatitis.

Aim

The aim of this pilot study was to evaluate the association between the expression of CD23 molecule on B cells and on their subsets (memory, naive, switched, non-switched, and total B lymphocytes) and the level of specific IgE to molecular components of mites in atopic dermatitis patients (with and without dupilumab therapy).

Methods

Forty-five patients suffering from atopic dermatitis were included: 32 patients without dupilumab treatment (10 men, 22 women, average age 35 years), 13 patients with dupilumab treatment (7 men, 6 women, average age 43.4 years) and 30 subjects as a control group (10 men, 20 women, average age 44.7 years). The serum level of the specific IgE was measured using the components resolved diagnostic microarray-based specific IgE detection assay ALEX2 Allergy Xplorer. In all included patients, the expression of CD23 molecule on B lymphocytes was evaluated with flow cytometry using monoclonal antibodies. For the statistical analysis of the association between expression of CD23 molecule on B lymphocytes and the level of specific IgE to molecular components of mites, we used non-parametric Kruskal-Wallis one-factor analysis of variance with post-hoc by Dunn's test with Bonferroni modification and the Spearman's rank correlation coefficient; for coefficients higher than 0.41, we report R2 (%, percent of Variation Explained).

Results

The association between the expression of CD23 molecule on B cells and the level of specific IgE to molecular components of mites was confirmed only in patients with dupilumab therapy. In these patients, the highest association was confirmed between the level of specific IgE to Der p 20 and expression of CD23 on switched B lymphocytes (in 48.9%). In patients without dupilumab, the association between the level of specific IgE to molecular components of mites and the expression of CD23 on B cells and on their subsets is low.

Conclusion

Further research is needed to fully understand the underlying mechanism of this phenomenon and its implications for the treatment of atopic dermatitis.

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特应性皮炎患者接受或未接受dupilumab治疗时螨类分子成分特异性IgE水平与B淋巴细胞CD23分子表达的关系

CD23分子通过刺激或抑制的方式调节IgE的合成。目前尚不清楚B淋巴细胞上CD23的表达是否与特应性皮炎患者的过敏原特异性IgE抗体水平有关。本初步研究的目的是评估特应性皮炎患者(接受和不接受dupilumab治疗)中B细胞及其亚群(记忆淋巴细胞、初始淋巴细胞、开关淋巴细胞、非开关淋巴细胞和总B淋巴细胞)上CD23分子表达与特异性IgE对螨分子成分水平之间的关系。方法纳入45例特应性皮炎患者:未接受dupilumab治疗的32例(男性10例，女性22例，平均年龄35岁)，接受dupilumab治疗的13例(男性7例，女性6例，平均年龄43.4岁)，对照组30例(男性10例，女性20例，平均年龄44.7岁)。血清特异性IgE水平采用成分分辨诊断微阵列特异性IgE检测试剂盒ALEX2 Allergy Xplorer检测。在所有纳入的患者中，使用单克隆抗体流式细胞术评估B淋巴细胞上CD23分子的表达。为统计分析B淋巴细胞CD23分子表达与螨类分子成分特异性IgE水平之间的关系，我们采用非参数Kruskal-Wallis单因素方差分析，事后采用Dunn's检验，Bonferroni修饰和Spearman's秩相关系数;对于系数高于0.41的，我们报告R2(%，变异解释百分比)。结果仅在dupilumab治疗的患者中，B细胞上CD23分子的表达与螨虫分子成分特异性IgE水平的相关性得到证实。在这些患者中，证实了特异性IgE对Der p20的水平与CD23在开关B淋巴细胞上的表达之间的最高相关性(48.9%)。在未使用dupilumab的患者中，螨虫分子成分特异性IgE水平与B细胞及其亚群上CD23表达之间的相关性较低。结论该现象的发生机制及其对特应性皮炎治疗的启示有待进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Translational Allergy Immunology and Microbiology-Immunology

CiteScore

7.50

自引率

4.50%

发文量

117

审稿时长

12 weeks

期刊介绍： Clinical and Translational Allergy, one of several journals in the portfolio of the European Academy of Allergy and Clinical Immunology, provides a platform for the dissemination of allergy research and reviews, as well as EAACI position papers, task force reports and guidelines, amongst an international scientific audience. Clinical and Translational Allergy accepts clinical and translational research in the following areas and other related topics: asthma, rhinitis, rhinosinusitis, drug hypersensitivity, allergic conjunctivitis, allergic skin diseases, atopic eczema, urticaria, angioedema, venom hypersensitivity, anaphylaxis, food allergy, immunotherapy, immune modulators and biologics, animal models of allergic disease, immune mechanisms, or any other topic related to allergic disease.

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