Analysis of microbial communities of ocular prostheses and anophthalmic sockets using 16S rRNA gene sequencing.

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biofouling Pub Date : 2023-05-01 Epub Date: 2023-07-20 DOI:10.1080/08927014.2023.2236562
L R Makrakis, V C Oliveira, E S Santos, C Nascimento, E Watanabe, A B Ribeiro, C H Silva-Lovato
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引用次数: 0

Abstract

Ocular prostheses are liable to host microbial adhesion, which can favor conjunctival inflammation. Knowing the microbiota of the ocular prosthesis and anophthalmic socket is important for predicting infection-related pathogens. This study evaluated the microbiota of the prostheses and anophthalmic sockets of six individuals by 16S rRNA sequencing. The microbial abundance and diversity were analyzed using the Operational Taxonomic Units at the genus level. Forty-seven phyla, capturing 1,258 named genera, were recorded in the sample set. In both sites, the most frequent genera were Fusobacterium, Staphylococcus, Prevotella, and Streptococcus. The microbial abundance was higher for the anophthalmic socket than for the prosthesis. Alpha diversity showed no significant differences in bacterial richness or diversity between sites. Although the microbial abundance in the anophthalmic socket was higher, both sites had similar microbiota with high diversity and low dominance among the genera.

应用16S rRNA基因测序分析义眼和无眼窝的微生物群落。
义眼易附着微生物,有利于结膜炎症。了解义眼和无眼窝的微生物群对于预测感染相关病原体很重要。本研究通过16S rRNA测序评估了6名患者的义眼和无眼窝的微生物群。使用属水平的操作分类单位分析微生物的丰度和多样性。样本集中记录了47个门,共有1258个命名属。在这两个地点,最常见的属是梭杆菌属、葡萄球菌属、普雷沃氏菌属和链球菌属。无眼窝的微生物丰度高于假体。阿尔法多样性在不同地点的细菌丰富度或多样性方面没有显著差异。尽管无眼窝中的微生物丰度较高,但两个位点的微生物群相似,属间多样性高,优势度低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biofouling
Biofouling 生物-海洋与淡水生物学
CiteScore
5.00
自引率
7.40%
发文量
57
审稿时长
1.7 months
期刊介绍: Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.
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