Toxicity of Ulva lactuca and green fabricated silver nanoparticles against mosquito vectors and their impact on the genomic DNA of the dengue vector Aedes aegypti.

IF 3.8 4区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

IET nanobiotechnology Pub Date : 2022-06-01 DOI:10.1049/nbt2.12082

Al Thabiani Aziz

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引用次数: 4

Abstract

Marine seaweeds are known to have a potential role against microbial and pesticidal activities. Ulva lactuca, a green macroalgae extract analysed through gas chromatography mass spectrometry reveals 31 compounds. Resistance of mosquito vectors to synthetic insecticides remains a major problem. Discovering and applying natural agents to act against disease vectors is challenging. The activities of the extract and nano-fabricated green synthesised silver nanoparticles were checked for use against Aedes aegypti and Culex pipiens. The crude extract and synthesised silver nanoparticles exhibited a notable larvicidal effect, and very effective inhibition of pupal and adult emergence. Inhibition of adult emergence of Ae.aegypti was 97.7% and in Cu.pipiens, it was 93.3%. Our genotypic study of Deoxyribonucleic acid from treated larvae utilising random primers MA-09, MA-12 and MA-26 revealed damaged nucleotide sequences when compared with the controls. The antimicrobial activity of both the extract and green synthesised nanomaterials showed prominent activity against pathogenic drug resistant bacteria. Our results contribute to further development of eco-friendly insecticides with lower cost of preparation. This could further contribute to further research helping future generations to be free from these deadly disease-causing vectors and pathogenic microbes.

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紫叶树和绿色纳米银对登革热媒介蚊子的毒性及其对埃及伊蚊基因组DNA的影响。

众所周知，海洋海藻具有抗微生物和杀虫活性的潜在作用。Ulva lactuca是一种绿色的大型藻类提取物，通过气相色谱-质谱分析发现了31种化合物。蚊媒对合成杀虫剂的抗药性仍然是一个主要问题。发现和应用自然媒介来对付病媒是一项挑战。研究了该提取物和纳米绿色合成银纳米颗粒对埃及伊蚊和库蚊的活性。粗提物和合成纳米银具有显著的杀虫效果，对蛹和成虫羽化有很好的抑制作用。抑制伊蚊成虫羽化的研究。埃及伊蚊占97.7%;库蚊占93.3%。我们利用随机引物MA-09、MA-12和MA-26对处理后幼虫的脱氧核糖核酸进行基因型研究，发现与对照组相比，核苷酸序列受损。提取物和绿色合成纳米材料的抗菌活性均显示出对致病性耐药菌的显著活性。我们的研究结果有助于进一步开发低成本的生态友好型杀虫剂。这可以进一步促进进一步的研究，帮助后代摆脱这些致命的致病媒介和致病微生物。

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来源期刊

IET nanobiotechnology 工程技术-纳米科技

CiteScore

6.20

自引率

4.30%

发文量

审稿时长

1 months

期刊介绍： Electrical and electronic engineers have a long and illustrious history of contributing new theories and technologies to the biomedical sciences. This includes the cable theory for understanding the transmission of electrical signals in nerve axons and muscle fibres; dielectric techniques that advanced the understanding of cell membrane structures and membrane ion channels; electron and atomic force microscopy for investigating cells at the molecular level. Other engineering disciplines, along with contributions from the biological, chemical, materials and physical sciences, continue to provide groundbreaking contributions to this subject at the molecular and submolecular level. Our subject now extends from single molecule measurements using scanning probe techniques, through to interactions between cells and microstructures, micro- and nano-fluidics, and aspects of lab-on-chip technologies. The primary aim of IET Nanobiotechnology is to provide a vital resource for academic and industrial researchers operating in this exciting cross-disciplinary activity. We can only achieve this by publishing cutting edge research papers and expert review articles from the international engineering and scientific community. To attract such contributions we will exercise a commitment to our authors by ensuring that their manuscripts receive rapid constructive peer opinions and feedback across interdisciplinary boundaries. IET Nanobiotechnology covers all aspects of research and emerging technologies including, but not limited to: Fundamental theories and concepts applied to biomedical-related devices and methods at the micro- and nano-scale (including methods that employ electrokinetic, electrohydrodynamic, and optical trapping techniques) Micromachining and microfabrication tools and techniques applied to the top-down approach to nanobiotechnology Nanomachining and nanofabrication tools and techniques directed towards biomedical and biotechnological applications (e.g. applications of atomic force microscopy, scanning probe microscopy and related tools) Colloid chemistry applied to nanobiotechnology (e.g. cosmetics, suntan lotions, bio-active nanoparticles) Biosynthesis (also known as green synthesis) of nanoparticles; to be considered for publication, research papers in this area must be directed principally towards biomedical research and especially if they encompass in vivo models or proofs of concept. We welcome papers that are application-orientated or offer new concepts of substantial biomedical importance Techniques for probing cell physiology, cell adhesion sites and cell-cell communication Molecular self-assembly, including concepts of supramolecular chemistry, molecular recognition, and DNA nanotechnology Societal issues such as health and the environment Special issues. Call for papers: Smart Nanobiosensors for Next-generation Biomedical Applications - https://digital-library.theiet.org/files/IET_NBT_CFP_SNNBA.pdf Selected extended papers from the International conference of the 19th Asian BioCeramic Symposium - https://digital-library.theiet.org/files/IET_NBT_CFP_ABS.pdf