Božena Klodová, David Potěšil, Lenka Steinbachová, Christos Michailidis, Ann-Cathrin Lindner, Dieter Hackenberg, Jörg D Becker, Zbyněk Zdráhal, David Twell, David Honys
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引用次数: 3
Abstract
Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronized gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model.
期刊介绍:
Plant Reproduction (formerly known as Sexual Plant Reproduction) is a journal devoted to publishing high-quality research in the field of reproductive processes in plants. Article formats include original research papers, expert reviews, methods reports and opinion papers. Articles are selected based on significance for the field of plant reproduction, spanning from the induction of flowering to fruit development. Topics incl … show all