Two novel symbiovars of Bradyrhizobium yuanmingense, americaense and caribense, the symbiovar tropici of Bradyrhizobium pachyrhizi and the symbiovar cajani of Bradyrhizobium cajani are microsymbionts of the legume Cajanus cajan in Dominican Republic

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2023-09-01 DOI:10.1016/j.syapm.2023.126454

José David Flores-Félix , Fernando Sánchez-Juanes , Juan Araujo , César Antonio Díaz-Alcántara , Encarna Velázquez , Fernando González-Andrés

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Abstract

Cajanus cajan L. (guandul) is commonly cultivated in Dominican Republic where this legume is a subsistence crop. Here we identified through MALDI-TOF MS several rhizobial strains nodulating C. cajan in two Dominican locations as Bradyrhizobium yuanmingense. The phylogenetic analysis of recA and glnII housekeeping genes showed that these strains belong to a wide cluster together with the type strain of B. yuanmingense and other C. cajan nodulating strains previously isolated in Dominican Republic. The comparison of genomes from strains representative of different lineages within this cluster support the existence of several genospecies within B. yuanmingense, which is the major microsymbiont of C. cajan in Dominican Republic where it is also nodulated by Bradyrhizobium cajani and Bradyrhizobium pachyrhizi. The analysis of the symbiotic nodC gene showed that the C. cajan nodulating strains from the B. yuanmingense complex belong to two clusters with less than 90% similarity between them. The strains from these two clusters showed nodC gene similarity values lower than 90% with respect to the remaining Bradyrhizobium symbiovars and then they correspond to two new symbiovars for which we propose the names americaense and caribense. The results of the nodC gene analysis also showed that C. cajan is nodulated by the symbiovar tropici, which has been found by first time in this work within the species Bradyrhizobium pachyrhizi. These results confirmed the high promiscuity degree of C. cajan, which is also nodulated by the symbiovar cajani of Bradyrhizobium cajani in Dominican Republic.

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远明慢生根瘤菌的两个新的共生体，美洲和加勒比，厚脊慢生根瘤杆菌的共生体tropici和卡氏慢生根瘤虫的共生体cajani是多米尼加共和国豆科植物Cajanus cajan的微共生体。

Cajanus cajan L.（guandul）通常在多米尼加共和国种植，这种豆类是一种自给作物。在这里，我们通过MALDI-TOF MS鉴定了在多米尼加的两个地方结瘤C.cajan的几种根瘤菌菌株，称为Bradyrhizobium yuanmingense。recA和glnII持家基因的系统发育分析表明，这些菌株与多米尼加共和国以前分离的B.yuanmingense型菌株和其他C.cajan结瘤菌株属于一个广泛的类群。对该集群中代表不同谱系的菌株的基因组进行比较，支持了圆明根B.yuamingense中存在几个基因种，圆明根是多米尼加共和国C.cajan的主要微共生体，在那里它也被Bradyrhizobium cajani和Bradyrhizabium pachyrhizi结瘤。对共生nodC基因的分析表明，来自圆明园B.mingense复合体的C.cajan结瘤菌株属于两个聚类，它们之间的相似性小于90%。来自这两个集群的菌株相对于剩余的慢生根瘤菌共生体表现出低于90%的nodC基因相似性值，然后它们对应于我们提出的名称为americaense和caribeense的两个新共生体。nodC基因分析的结果还表明，C.cajan是由共生体tropici结瘤的，该共生体在本工作中首次在Bradyrhizobium pachyrhizi物种中发现。这些结果证实了C.cajan的高度混杂程度，多米尼加共和国的Bradyrhizobium cajani共生体cajani也使其结瘤。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567