Isolation, Characterization, and Comparative Genomics of the Novel Potential Probiotics from Canine Feces.

IF 4.2 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Science of Animal Resources Pub Date : 2023-07-01 DOI:10.5851/kosfa.2023.e28

Ngamlak Foongsawat, Sirinthorn Sunthornthummas, Kwannan Nantavisai, Komwit Surachat, Achariya Rangsiruji, Siriruk Sarawaneeyaruk, Kedvadee Insian, Sirapan Sukontasing, Nuttika Suwannasai, Onanong Pringsulaka

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引用次数: 3

Abstract

Lactic acid bacteria (LAB) are commonly used as probiotics; however, not all LAB strains have the same beneficial effects. To successfully use LAB as probiotics in canines, LAB species should originate from the canine intestinal tract as they display host specificity. The objective of this study was to investigate the phenotypic and genomic traits of potential probiotic LAB isolated from canine fecal samples. Twenty LAB samples were evaluated for their potential probiotic characteristics including resistance to low pH, bile salts, hydrophobicity, auto-aggregation, co-aggregation, adhesion to epithelia or mucosa, and production of inhibitory compounds. Additionally, we evaluated their safety and other beneficial effects on canine health, such as DPPH free radical scavenging, and β-galactosidase. Four strains demonstrated potential probiotic characteristics and were selected: Enterococcus hirae Pom4, Limosilactobacillus fermentum Pom5, Pediococcus pentosaceus Chi8, and Ligilactobacillus animalis FB2. Safety evaluations showed that all strains lacked hemolytic activity, could not produce biogenic amines, and did not carry any pathogenic genes. In addition, L. fermentum Pom5 and P. pentosaceus Chi8 displayed susceptibility to all antibiotics and concordant with the absence of antibiotic resistance genes. Based on their phenotypic and genomic characteristics, L. fermentum Pom5 and P. pentosaceus Chi8 were identified as potential probiotic candidates for canines.

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犬粪中新型潜在益生菌的分离、鉴定和比较基因组学研究。

乳酸菌(LAB)是常用的益生菌;然而，并非所有的乳酸菌菌株都具有相同的有益效果。为了成功地将乳酸菌用作犬体内的益生菌，乳酸菌必须来自犬肠道，因为它们具有宿主特异性。本研究的目的是研究从犬粪便样本中分离的潜在益生菌乳酸菌的表型和基因组特征。对20个LAB样品的潜在益生菌特性进行了评估，包括对低pH值、胆盐、疏水性、自聚集、共聚集、对上皮或粘膜的粘附以及抑制化合物的产生。此外，我们还评估了它们的安全性和对犬健康的其他有益作用，如清除DPPH自由基和β-半乳糖苷酶。筛选出4株具有潜在益生菌特性的菌株，分别为:hiaenterococcus Pom4、Limosilactobacillus fermentum Pom5、pedococcus pentosaceus Chi8、liilactobacillus animalis FB2。安全性评价表明，所有菌株缺乏溶血活性，不能产生生物胺，不携带任何致病基因。此外，L. fermentum Pom5和P. pentosaceus Chi8对所有抗生素均表现出敏感性，与抗生素耐药基因的缺失一致。基于它们的表型和基因组特征，我们确定了L. fermentum Pom5和P. pentosaceus Chi8是潜在的犬用益生菌候选菌。

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来源期刊

Food Science of Animal Resources Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

6.70

自引率

6.70%

发文量

期刊介绍： Food Science of Animal Resources (Food Sci. Anim. Resour.) is an international, peer-reviewed journal publishing original research and review articles on scientific and technological aspects of chemistry, biotechnology, processing, engineering, and microbiology of meat, egg, dairy, and edible insect/worm products.