Perfusion in vivo bioreactor promotes regeneration of vascularized tissue-engineered bone.

IF 2.4 4区医学 Q4 CELL & TISSUE ENGINEERING

Regenerative medicine Pub Date : 2023-09-01 DOI:10.2217/rme-2023-0101

Peng Liu, Jian Wang, Yun Xue, Lei Zou, Yongzheng Tian, Ruilong Sun, Wenhua Zhang, Yunfei Li, Lijun Lv, Qiuming Gao, Bo Fan

引用次数: 0

Abstract

Aim: This study improved the in vivo bioreactor (IVB) for bone regeneration by enhancing stem cell survival and promoting vascularized tissue-engineered bone. Methods: 12 New Zealand rabbits received β-TCP scaffolds with rabbit bone mesenchymal stem cells (BMSCs) implanted. Perfusion IVB with a perfusion electronic pump was compared with the control group using micro-CT, Microfil perfusion, histological staining and RT-PCR for gene expression. Results: Perfusion IVB demonstrated good biocompatibility, increased neoplastic bone tissue, neovascularization and upregulated osteogenic and angiogenesis-related genes in rabbits (p < 0.05). Conclusion: Perfusion IVB holds promise for bone regeneration and tissue engineering in orthopedics and maxillofacial surgery.

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体内生物反应器灌注促进血管化组织工程骨的再生。

目的:通过提高干细胞存活率和促进血管化组织工程骨的形成，改进体内生物反应器(IVB)的骨再生功能。方法:12只新西兰兔植入兔骨间充质干细胞(BMSCs)的β-TCP支架。采用micro-CT、Microfil灌注、组织学染色及RT-PCR检测基因表达，将灌注电子泵灌注IVB与对照组进行比较。结果:灌注IVB在家兔中表现出良好的生物相容性，肿瘤骨组织、新生血管增加，成骨和血管生成相关基因上调(p)。结论:灌注IVB在骨科和颌面外科的骨再生和组织工程中具有前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regenerative medicine 医学-工程：生物医学

CiteScore

4.20

自引率

3.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization. Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community. Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.