Hydrolyzed Collagen Powder Dressing Improves Wound Inflammation, Perfusion, and Breaking Strength of Repaired Tissue.

IF 5.8 3区医学 Q1 DERMATOLOGY

Advances in wound care Pub Date : 2024-02-01 Epub Date: 2023-09-13 DOI:10.1089/wound.2023.0065

Manishekhar Kumar, Pradipta Banerjee, Amitava Das, Kanhaiya Singh, Tanner Guith, Sedat Kacar, Karthik Gourishetti, Chandan K Sen, Sashwati Roy, Savita Khanna

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引用次数: 0

Abstract

Objective: Hydrolyzed collagen-based matrices are widely used as wound care dressings. Information on the mechanism of action of such dressings is scanty. The objective of this study was to test the effect of a specific hydrolyzed collagen powder (HCP), which is extensively used for wound care management in the United States. Approach: The effects of HCP on resolution of wound inflammation, perfusion, closure, and breaking strength of the repaired skin were studied in an experimental murine model. Results: In early (day 7) inflammatory phase of wound macrophages, HCP treatment boosted phagocytosis and efferocytosis of wound-site macrophages. In these cells, inducible reactive oxygen species were also higher on day (d) 7. HCP treatment potentiated the expression of anti-inflammatory interleukin (IL)-10 cytokine and proangiogenic vascular endothelial growth factor (VEGF) production. Excisional wounds dressed with HCP showed complete closure on day 21, while the control wounds remained open. HCP treatment also demonstrated improved quality of wound healing as marked by the improved breaking strength of the closed wound tissue/repaired skin. Innovation: These data represent first evidence on the mechanism of action of clinically used HCP. Conclusion: HCP dressing favorably influenced both wound inflammation and vascularization. Improved breaking strength of HCP-treated repaired skin lays the rationale for future studies testing the hypothesis that HCP-treated closed wounds would show fewer recurrences.

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水解胶原蛋白粉末敷料改善伤口炎症、灌注和修复组织的断裂强度。

目的:水解胶原基基质广泛应用于伤口护理敷料。关于这种敷料的作用机制的资料很少。本研究的目的是测试特异性水解胶原蛋白粉(HCP)的效果，HCP在美国广泛用于伤口护理管理。方法:采用小鼠实验模型，研究HCP对创面炎症消退、灌注、闭合及修复后皮肤断裂强度的影响。结果:在创面巨噬细胞炎症早期(第7天)，HCP处理促进了创面巨噬细胞的吞噬和efferocytic。在这些细胞中，诱导性活性氧在第7天也较高。HCP治疗增强了抗炎白细胞介素(IL)-10细胞因子的表达和促血管生成血管内皮生长因子(VEGF)的产生。用HCP包扎的切除伤口在第21天完全闭合，而对照组伤口保持开放。HCP治疗还显示出伤口愈合质量的改善，其标志是闭合的伤口组织/修复的皮肤的断裂强度得到改善。创新:这些数据首次证明了临床使用的HCP的作用机制。结论:HCP敷料对创面炎症和血管形成均有良好的影响。hcp处理的修复皮肤的断裂强度提高为未来的研究奠定了基础，验证了hcp处理的闭合伤口复发较少的假设。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in wound care Medicine-Emergency Medicine

CiteScore

12.10

自引率

4.10%

发文量

期刊介绍： Advances in Wound Care rapidly shares research from bench to bedside, with wound care applications for burns, major trauma, blast injuries, surgery, and diabetic ulcers. The Journal provides a critical, peer-reviewed forum for the field of tissue injury and repair, with an emphasis on acute and chronic wounds. Advances in Wound Care explores novel research approaches and practices to deliver the latest scientific discoveries and developments. Advances in Wound Care coverage includes: Skin bioengineering, Skin and tissue regeneration, Acute, chronic, and complex wounds, Dressings, Anti-scar strategies, Inflammation, Burns and healing, Biofilm, Oxygen and angiogenesis, Critical limb ischemia, Military wound care, New devices and technologies.