New Potential MicroRNA Biomarkers in Human Immunodeficiency Virus Elite Controllers, Human Immunodeficiency Virus Infections, and Coinfections with Hepatitis B Virus or Hepatitis C Virus.

IF 3.2 4区医学 Q3 VIROLOGY

Intervirology Pub Date : 2023-01-01 Epub Date: 2023-09-12 DOI:10.1159/000533595

Bashdar Mahmud Hussen, Majid Noori, Babak Sayad, Maryam Ebadi Fard Azar, Javid Sadri Nahand, Mobina Bayat, Farhad Babaei, Romina Karampour, Farah Bokharaei-Salim, Hamed Mirzaei, Mohsen Moghoofei, Hossein Bannazadeh Baghi

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引用次数: 0

Abstract

Introduction: This research aimed to evaluate the specific microRNA (miRNA) including miR-17-5p, miRN-140-3p miR-191-5p, miR-200c-3p, and miR-N367 and cellular factors (p21, SDF-1, XCL1, CCL-2, and IL-2) in controlling replication of human immunodeficiency virus (HIV) in ECs.

Methods: The expression of miRNAs was assessed between healthy control groups and patient groups including ART-naïve HIV, HIV ART, ECs, and coinfection (HIV-HBV and HIV-HCV) via real-time PCR technique. Besides, the expression level of the nef gene and cellular factors were assessed by the ELISA method. The differences in the level of cellular factors and selected miRNAs between study groups were analyzed using the Kruskal-Wallis H or one-way ANOVA test. In addition, the potential of selected miRNAs as biomarkers for discriminating study groups was assessed by the receiver-operator characteristic (ROC) curve analysis.

Results: Some miRNAs in ECs, HIV ART, and healthy controls have similar expression patterns, whereas a miRNA expression profile of patient groups significantly differed compared to EC and control groups. According to ROC curve analyses, the miR-17-5p, miR-140-3p miR-191-5p, miR-200c-3p, and miR-N367 can be served as biomarkers for discriminating ECs from ART-naïve HIV-infected groups. There was a significant correlation between some miRNAs and cellular factors/the viral load as well.

Conclusion: This report demonstrated a differentiation in the expression of selected immunological factors and cellular/viral miRNAs in ECs compared to other patient groups. Some miRNAs and cellular factors are involved in the viral replication control, immune response/modulation and can be used as biomarkers for diagnosis of ECs and differentiation with other groups. Differential expression of these miRNAs and cellular factors in different stages of HIV infection can help in finding novel ways for infection control.

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人类免疫缺陷病毒精英控制者、人类免疫缺陷病毒感染以及乙型肝炎病毒或丙型肝炎病毒合并感染中新的潜在微RNA生物标记物。

引言本研究旨在评估特定微RNA（miRNA），包括miR-17-5p、miRN-140-3p、miR-191-5p、miR-200c-3p和miR-N367，以及细胞因子（p21、SDF-1、XCL1、CCL-2和IL-2）在控制人类免疫缺陷病毒（HIV）在ECs中复制中的作用：方法：通过实时 PCR 技术评估健康对照组和患者组（包括抗逆转录病毒疗法（ART）无效 HIV、抗逆转录病毒疗法（ART）无效 HIV、ECs 和合并感染（HIV-HBV 和 HIV-HCV））之间 miRNAs 的表达。此外，还采用 ELISA 方法评估了 nef 基因和细胞因子的表达水平。采用 Kruskal-Wallis H 或单向方差分析检验分析研究组间细胞因子和所选 miRNA 水平的差异。此外，研究人员还利用接收器-操作者特征（ROC）曲线分析法评估了所选 miRNA 作为生物标志物用于区分研究组的潜力：结果：一些miRNA在EC、HIV ART和健康对照组中有相似的表达模式，而患者组的miRNA表达谱与EC组和对照组相比有显著差异。根据 ROC 曲线分析，miR-17-5p、miR-140-3p、miR-191-5p、miR-200c-3p 和 miR-N367 可作为生物标记物，用于区分 ECs 和抗逆转录病毒疗法无效的 HIV 感染者。一些 miRNA 与细胞因素/病毒载量之间也存在明显的相关性：本报告显示，与其他患者群体相比，ECs 中某些免疫因子和细胞/病毒 miRNAs 的表达存在差异。一些 miRNA 和细胞因子参与病毒复制控制、免疫反应/调节，可用作诊断心肌梗死的生物标志物，并与其他组别进行区分。这些 miRNA 和细胞因子在 HIV 感染不同阶段的差异表达有助于找到控制感染的新方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Intervirology 医学-病毒学

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.