The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses.

IF 9.5 1区 生物学 Q1 MYCOLOGY
Persoonia Pub Date : 2021-12-01 Epub Date: 2021-10-01 DOI:10.3767/persoonia.2021.47.04
N Kobmoo, N Arnamnart, W Pootakham, C Sonthirod, A Khonsanit, W Kuephadungphan, R Suntivich, O V Mosunova, T Giraud, J J Luangsa-Ard
{"title":"The integrative taxonomy of <i>Beauveria asiatica</i> and <i>B. bassiana</i> species complexes with whole-genome sequencing, morphometric and chemical analyses.","authors":"N Kobmoo, N Arnamnart, W Pootakham, C Sonthirod, A Khonsanit, W Kuephadungphan, R Suntivich, O V Mosunova, T Giraud, J J Luangsa-Ard","doi":"10.3767/persoonia.2021.47.04","DOIUrl":null,"url":null,"abstract":"<p><p>Fungi are rich in complexes of cryptic species that need a combination of different approaches to be delimited, including genomic information. <i>Beauveria</i> (<i>Cordycipitaceae</i>, <i>Hypocreales</i>) is a well-known genus of entomopathogenic fungi, used as a biocontrol agent. In this study we present a polyphasic taxonomy regarding two widely distributed complexes of <i>Beauveria</i>: <i>B. asiatica</i> and <i>B. bassiana</i> s.lat. Some of the genetic groups as previously detected within both taxa were either confirmed or fused using population genomics. High levels of divergence were found between two clades in <i>B. asiatica</i> and among three clades in <i>B. bassiana</i>, supporting their subdivision as distinct species. Morphological examination focusing on the width and the length of phialides and conidia showed no difference among the clades within <i>B. bassiana</i> while conidial length was significantly different among clades within <i>B. asiatica</i>. The secondary metabolite profiles obtained by liquid chromatography-mass spectrometry (LC-MS) allowed a distinction between <i>B. asiatica</i> and <i>B. bassiana</i>, but not between the clades therein. Based on these genomic, morphological, chemical data, we proposed a clade of <i>B. asiatica</i> as a new species, named <i>B. thailandica</i>, and two clades of <i>B. bassiana</i> to respectively represent <i>B. namnaoensis</i> and <i>B. neobassiana</i> spp. nov. Such closely related but divergent species with different host ranges have potential to elucidate the evolution of host specificity, with potential biocontrol application. <b>Citation</b>: Kobmoo N, Arnamnart N, Pootakham W, et al. 2021. The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses. Persoonia 47: 136-150. https://doi.org/10.3767/persoonia.2021.47.04.</p>","PeriodicalId":20014,"journal":{"name":"Persoonia","volume":null,"pages":null},"PeriodicalIF":9.5000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486633/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Persoonia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3767/persoonia.2021.47.04","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/10/1 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Fungi are rich in complexes of cryptic species that need a combination of different approaches to be delimited, including genomic information. Beauveria (Cordycipitaceae, Hypocreales) is a well-known genus of entomopathogenic fungi, used as a biocontrol agent. In this study we present a polyphasic taxonomy regarding two widely distributed complexes of Beauveria: B. asiatica and B. bassiana s.lat. Some of the genetic groups as previously detected within both taxa were either confirmed or fused using population genomics. High levels of divergence were found between two clades in B. asiatica and among three clades in B. bassiana, supporting their subdivision as distinct species. Morphological examination focusing on the width and the length of phialides and conidia showed no difference among the clades within B. bassiana while conidial length was significantly different among clades within B. asiatica. The secondary metabolite profiles obtained by liquid chromatography-mass spectrometry (LC-MS) allowed a distinction between B. asiatica and B. bassiana, but not between the clades therein. Based on these genomic, morphological, chemical data, we proposed a clade of B. asiatica as a new species, named B. thailandica, and two clades of B. bassiana to respectively represent B. namnaoensis and B. neobassiana spp. nov. Such closely related but divergent species with different host ranges have potential to elucidate the evolution of host specificity, with potential biocontrol application. Citation: Kobmoo N, Arnamnart N, Pootakham W, et al. 2021. The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses. Persoonia 47: 136-150. https://doi.org/10.3767/persoonia.2021.47.04.

Abstract Image

Abstract Image

Abstract Image

利用全基因组测序、形态计量学和化学分析对亚洲贝维氏菌(Beauveria asiatica)和巴西贝维氏菌(B. bassiana)物种复合体进行综合分类。
真菌中存在丰富的隐性物种群,需要结合不同的方法(包括基因组信息)才能对其进行划分。Beauveria(虫草科,Hypocreales)是一个著名的昆虫病原真菌属,被用作生物控制剂。在本研究中,我们对两种广泛分布的蒲公英复合菌进行了多相分类:B. asiatica 和 B. bassiana s.lat.以前在这两个类群中检测到的一些基因群通过群体基因组学得到了证实或融合。在 B. asiatica 的两个支系之间和 B. bassiana 的三个支系之间发现了高度的分化,支持将它们细分为不同的物种。形态学检查的重点是噬菌体和分生孢子的宽度和长度,结果表明 B. bassiana 内部各支系之间没有差异,而 B. asiatica 内部各支系之间的分生孢子长度有显著差异。通过液相色谱-质谱法(LC-MS)获得的次生代谢物图谱可以区分 B. asiatica 和 B. bassiana,但不能区分其中的支系。根据这些基因组、形态学和化学数据,我们提议将 B. asiatica 的一个支系作为一个新种,命名为 B. thailandica,并将 B. bassiana 的两个支系分别代表 B. namnaoensis 和 B. neobassiana spp.这些亲缘关系密切但寄主范围不同的物种有可能阐明寄主特异性的进化,具有潜在的生物防治应用价值。引用:Kobmoo N, Arnamnart N, Pootakham W, et al.利用全基因组测序、形态计量学和化学分析对亚西亚真菌(Beauveria asiatica)和巴西真菌(B. bassiana)物种复合体进行综合分类。Persoonia 47: 136-150. https://doi.org/10.3767/persoonia.2021.47.04.
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Persoonia
Persoonia MYCOLOGY-
CiteScore
17.70
自引率
5.50%
发文量
10
审稿时长
3 months
期刊介绍: Persoonia aspires to publish papers focusing on the molecular systematics and evolution of fungi. Additionally, it seeks to advance fungal taxonomy by employing a polythetic approach to elucidate the genuine phylogeny and relationships within the kingdom Fungi. The journal is dedicated to disseminating high-quality papers that unravel both known and novel fungal taxa at the DNA level. Moreover, it endeavors to provide fresh insights into evolutionary processes and relationships. The scope of papers considered encompasses research articles, along with topical and book reviews.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信