Impact of administering umbilical cord-derived mesenchymal stem cells to cynomolgus monkeys with endometriosis.

IF 2.7 3区医学 Q2 OBSTETRICS & GYNECOLOGY

Reproductive Medicine and Biology Pub Date : 2023-09-08 eCollection Date: 2023-01-01 DOI:10.1002/rmb2.12540

Shunichiro Tsuji, Takeo Mukai, Hideaki Tsuchiya, Chizuru Iwatani, Akiko Nakamura, Tokiko Nagamura-Inoue, Takashi Murakami

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Abstract

Purpose: This study aimed to explore whether umbilical cord-derived mesenchymal stem cells (UC-MSCs) could be used as a therapeutic resource for endometriosis.

Methods: Of seven cynomolgus monkeys with endometriosis, five were administered UC-MSCs (intervention group) and two were administered saline (control group). First, intravenous US-MSC treatment was administered for three months. Second, weekly intravenous US-MSC administration combined with monthly intraperitoneal US-MSC administration was conducted for 3 months. Finally, weekly intraperitoneal US-MSC administration was conducted for 3 months. The dose of UC-MSCs was set to 2 × 10⁶ cells/kg for all administration routes. Laparoscopic findings and serum cancer antigen 125 (CA125) levels were also evaluated. The Revised American Society for Reproductive Medicine classification was used for laparoscopic evaluation.

Results: Laparoscopic findings showed exacerbation of endometriosis after intraperitoneal UC-MSC administration, although no changes were observed in the control group. Intravenous UC-MSC administration decreased the level of CA125 in all monkeys; however, the difference was not significant. Intraperitoneal UC-MSC administration significantly exacerbated endometriosis compared with intravenous administration (p = 0.02).

Conclusions: This study revealed that intraperitoneal UC-MSC administration exacerbates endometriosis in a nonhuman primate model of the disease.

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对患有子宫内膜异位症的绒猴施用脐带间充质干细胞的影响。

目的：本研究旨在探讨脐带间充质干细胞（UC-MSCs）是否可用作子宫内膜异位症的治疗资源：方法：在7只患有子宫内膜异位症的绒猴中，5只注射了UC-间充质干细胞（干预组），2只注射了生理盐水（对照组）。首先，进行为期三个月的静脉注射 US-间充质干细胞治疗。其次，每周静脉注射 US-间充质干细胞，同时每月腹腔注射 US-间充质干细胞，为期 3 个月。最后，每周一次腹腔注射 US-间充质干细胞，为期 3 个月。所有给药途径的USC-间充质干细胞剂量均为2×106个细胞/千克。此外，还对腹腔镜检查结果和血清癌抗原125（CA125）水平进行了评估。腹腔镜评估采用了美国生殖医学会修订的分类标准：结果：腹腔镜检查结果显示，腹腔注射 UC-间充质干细胞后，子宫内膜异位症加重，而对照组未见变化。静脉注射 UC-MSC 降低了所有猴子的 CA125 水平，但差异不显著。与静脉注射相比，腹腔注射 UC-MSC 会明显加重子宫内膜异位症（p = 0.02）：这项研究表明，在非人灵长类动物模型中，腹腔注射 UC 间充质干细胞会加重子宫内膜异位症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive Medicine and Biology Multiple-

CiteScore

5.70

自引率

5.90%

发文量

审稿时长

20 weeks

期刊介绍： Reproductive Medicine and Biology (RMB) is the official English journal of the Japan Society for Reproductive Medicine, the Japan Society of Fertilization and Implantation, the Japan Society of Andrology, and publishes original research articles that report new findings or concepts in all aspects of reproductive phenomena in all kinds of mammals. Papers in any of the following fields will be considered: andrology, endocrinology, oncology, immunology, genetics, function of gonads and genital tracts, erectile dysfunction, gametogenesis, function of accessory sex organs, fertilization, embryogenesis, embryo manipulation, pregnancy, implantation, ontogenesis, infectious disease, contraception, etc.