Osteochondral Repair with Autologous Cartilage Transplantation with or without Bone Grafting: A Short Pilot Study in Mini-Pigs.

IF 2.7 4区医学 Q1 ORTHOPEDICS

CARTILAGE Pub Date : 2025-03-01 Epub Date: 2023-09-12 DOI:10.1177/19476035231199442

Dong Woo Shim, Kyoung-Mi Lee, Donghyun Lee, Jun Sik Kim, Yeon Seop Jung, Sung Suk Oh, Si Wook Lee, Jin Woo Lee, Bom Soo Kim

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引用次数: 0

Abstract

Objective: Treatment strategies for osteochondral defects, for which particulated autologous cartilage transplantation (PACT) is an emerging treatment strategy, aim to restore the structure and function of the hyaline cartilage. Herein, we compared the efficacy of PACT with control or human transforming growth factor-β (rhTGF-β), and clarified the necessity of bone graft (BG) with PACT to treat shallow osteochondral defects in a porcine model.

Design: Two skeletally mature male micropigs received 4 osteochondral defects in each knee. The 16 defects were randomized to (1) empty control, (2) PACT, (3) PACT with BG, or (4) rhTGF-β. Animals were euthanized after 2 months and histomorphometry, immunofluorescence analysis, semiquantitative evaluation (O'Driscoll score), and magnetic resonance observation of cartilage repair tissue (MOCART) score were performed.

Results: Hyaline cartilages, glycosaminoglycan synthesis, and collagen type II staining were more abundant in the PACT than in the control and rhTGF-β groups. The O'Driscoll score was significantly different between groups (P < 0.001), with both PACT groups showing superiority (P = 0.002). PACT had the highest score (P = 0.002), with improved restoration of subchondral bone compared with PACT with BG. The MOCART score showed significant differences between groups (P = 0.021); MOCART and O'Driscoll scores showed high correlation (r = 0.847, P < 0.001).

Conclusion: Treatment of osteochondral defects with PACT improved tissue quality compared with that with control or rhTGF-β in a porcine model. BG, in addition to PACT, may be unnecessary for shallow osteochondral defects. Clinical Relevance. BG may not be necessary while performing PACT.

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自体软骨移植伴或不伴骨移植的骨软骨修复：一项小型猪的简短初步研究。

目的：修复透明软骨的结构和功能是骨软骨缺损的治疗策略，而微粒自体软骨移植是一种新兴的治疗策略。在此，我们比较了PACT与对照或人转化生长因子-β (rhTGF-β)的疗效，并明确了PACT骨移植（BG）治疗猪模型浅骨软骨缺损的必要性。设计：两只骨骼成熟的雄性微型猪，每只膝盖各有4个骨软骨缺损。16个缺陷被随机分为(1)空白对照，(2)PACT,(3)与BG的PACT，或(4)rhTGF-β。2个月后安乐死，进行组织形态学测定、免疫荧光分析、半定量评价（O’driscoll评分）、磁共振观察软骨修复组织（MOCART）评分。结果：PACT组的透明软骨、糖胺聚糖合成和II型胶原染色较对照组和rhTGF-β组丰富。O’driscoll评分组间差异有统计学意义（P < 0.001）， PACT组和PACT组均有优势（P = 0.002）。PACT评分最高（P = 0.002），与PACT联合BG相比，其软骨下骨的修复效果更好。MOCART评分组间差异有统计学意义（P = 0.021）；MOCART与O'Driscoll评分呈高度相关（r = 0.847, P < 0.001）。结论：与对照组和rhTGF-β相比，PACT治疗猪骨软骨缺损能改善组织质量。对于浅层骨软骨缺损，除PACT外，可能不需要BG。临床相关性。执行PACT时可能不需要BG。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

CARTILAGE ORTHOPEDICS-

CiteScore

6.90

自引率

7.10%

发文量

期刊介绍： CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.