The neurobehavioral impacts of typical antibiotics toward zebrafish larvae

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere Pub Date : 2023-11-01 DOI:10.1016/j.chemosphere.2023.139829

Yining Zhang , Xue Li , Zaiteng Liu , Xiaoyu Zhao , Lu Chen , Guijie Hao , Xueping Ye , Shunlong Meng , Guohua Xiao , Jiandong Mu , Xiyan Mu , Jing Qiu , Yongzhong Qian

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引用次数: 1

Abstract

Due to the widely usage in livestock, aquaculture and clinics, antibiotic residues are existed in aqueous environments and their potential toxicity to aquatic organisms is concerning. Here, we used zebrafish as the model to investigate the neurotoxicity and involved mechanism of seven antibiotics that were frequently detected in surface waters. The results revealed that the short-term exposure to clarithromycin (CLA), chlortetracycline (CTC) and roxithromycin (ROX) induced behavioral effects, with effective concentration of 1 μg/L (CTC and ROX) and 100 μg/L (CLA, CTC and ROX) respectively. A significant decrease in the travel distance and velocity as well as an increase in turn angle was measured. TUNEL assay identified increased cell apoptosis in brain sections of larvae exposed to three neurotoxic antibiotics, which raised the possibility that the behavioral symptoms were associated with neural damage. Transcriptome sequencing showed that the three antibiotics could affect the nervous system of zebrafish including the alteration of synaptogenesis and neurotransmission. Additionally, ROX and CTC affected pathways involved in mitochondrial stress response and endocrine system in zebrafish larvae. Besides, BDNF, ASCL1, and CREBBP are potential upstream regulatory factors that mediated these impacts. These findings indicated that exposure of CTC, ROX and CLA may cause abnormal behavior toward zebrafish larvae under environmental relevant concentration and revealed the potential role of neural cell apoptosis and synaptogenesis signaling in mediating this effect.

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典型抗生素对斑马鱼幼虫的神经行为影响。

由于抗生素在畜牧业、水产养殖和诊所中的广泛应用，抗生素残留存在于水环境中，其对水生生物的潜在毒性令人担忧。在这里，我们以斑马鱼为模型，研究了在地表水中经常检测到的七种抗生素的神经毒性和相关机制。结果表明，短期暴露于克拉霉素（CLA）、金霉素（CTC）和罗红霉素（ROX）可引起行为效应，有效浓度分别为1μg/L（CTC和ROX）和100μg/L（CLA、CTC和ROX）。测量到行驶距离和速度的显著减小以及转向角的增大。TUNEL检测发现，暴露于三种神经毒性抗生素的幼虫大脑切片中细胞凋亡增加，这增加了行为症状与神经损伤相关的可能性。转录组测序显示，这三种抗生素可以影响斑马鱼的神经系统，包括突触发生和神经传递的改变。此外，ROX和CTC影响斑马鱼幼虫线粒体应激反应和内分泌系统的途径。此外，BDNF、ASCL1和CREBBP是介导这些影响的潜在上游调节因子。这些发现表明，在环境相关浓度下，暴露于CTC、ROX和CLA可能导致对斑马鱼幼虫的异常行为，并揭示了神经细胞凋亡和突触发生信号在介导这种作用中的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemosphere 环境科学-环境科学

CiteScore

15.80

自引率

8.00%

发文量

4975

审稿时长

3.4 months

期刊介绍： Chemosphere, being an international multidisciplinary journal, is dedicated to publishing original communications and review articles on chemicals in the environment. The scope covers a wide range of topics, including the identification, quantification, behavior, fate, toxicology, treatment, and remediation of chemicals in the bio-, hydro-, litho-, and atmosphere, ensuring the broad dissemination of research in this field.