Kristina Snipaitiene, Birute Zablockiene, Rasa Sabaliauskaite, Kristina Zukauskaite, Elzbieta Matulyte, Tautvile Smalinskaite, Mindaugas Paulauskas, Rolandas Zablockis, Mantvydas Lopeta, Julius Gagilas, Alina Puriene, Ligita Jancoriene, Sonata Jarmalaite
{"title":"SARS-CoV-2 RT-qPCR Ct values in saliva and nasopharyngeal swab samples for disease severity prediction.","authors":"Kristina Snipaitiene, Birute Zablockiene, Rasa Sabaliauskaite, Kristina Zukauskaite, Elzbieta Matulyte, Tautvile Smalinskaite, Mindaugas Paulauskas, Rolandas Zablockis, Mantvydas Lopeta, Julius Gagilas, Alina Puriene, Ligita Jancoriene, Sonata Jarmalaite","doi":"10.1080/20002297.2023.2213106","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Comparison of clinical value of RT-qPCR-based SARS-CoV-2 tests performed on saliva samples (SSs) and nasopharyngeal swab samples (NPSs) for prediction of the COVID-19 disease severity.</p><p><strong>Methods: </strong>Three paired SSs and NPSs collected every 3 days from 100 hospitalised COVID-19 patients during 2020 Jul-2021 Jan were tested by RT-qPCR for the original SARS-CoV-2 virus and compared to 150 healthy controls. Cases were divided into mild+moderate (Cohort I, <i>N</i> = 47) and severe disease (Cohort II, <i>N</i> = 53) cohorts and compared.</p><p><strong>Results: </strong>SARS-CoV-2 was detected in 65% (91/140) vs. 53% (82/156) of NPSs and 49% (68/139) vs. 48% (75/157) of SSs collected from Cohort I and II, respectively, resulting in the total respective detection rates of 58% (173/296) vs. 48% (143/296) (<i>P</i> = 0.017). Ct values of SSs were lower than those of NPSs (mean Ct = 28.01 vs. 30.07, <i>P</i> = 0.002). Although Ct values of the first SSs were significantly lower in Cohort I than in Cohort II (<i>P</i> = 0.04), it became negative earlier (mean 11.7 vs. 14.8 days, <i>P</i> = 0.005). Multivariate Cox proportional hazards regression analysis showed that Ct value ≤30 from SSs was the independent predictor for severe COVID-19 (HR = 10.06, 95% CI: 1.84-55.14, <i>P</i> = 0.008).</p><p><strong>Conclusion: </strong>Salivary RT-qPCR testing is suitable for SARS-CoV-2 infection control, while simple measurement of Ct values can assist in prediction of COVID-19 severity.</p>","PeriodicalId":16598,"journal":{"name":"Journal of Oral Microbiology","volume":"15 1","pages":"2213106"},"PeriodicalIF":3.7000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10193917/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/20002297.2023.2213106","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Comparison of clinical value of RT-qPCR-based SARS-CoV-2 tests performed on saliva samples (SSs) and nasopharyngeal swab samples (NPSs) for prediction of the COVID-19 disease severity.
Methods: Three paired SSs and NPSs collected every 3 days from 100 hospitalised COVID-19 patients during 2020 Jul-2021 Jan were tested by RT-qPCR for the original SARS-CoV-2 virus and compared to 150 healthy controls. Cases were divided into mild+moderate (Cohort I, N = 47) and severe disease (Cohort II, N = 53) cohorts and compared.
Results: SARS-CoV-2 was detected in 65% (91/140) vs. 53% (82/156) of NPSs and 49% (68/139) vs. 48% (75/157) of SSs collected from Cohort I and II, respectively, resulting in the total respective detection rates of 58% (173/296) vs. 48% (143/296) (P = 0.017). Ct values of SSs were lower than those of NPSs (mean Ct = 28.01 vs. 30.07, P = 0.002). Although Ct values of the first SSs were significantly lower in Cohort I than in Cohort II (P = 0.04), it became negative earlier (mean 11.7 vs. 14.8 days, P = 0.005). Multivariate Cox proportional hazards regression analysis showed that Ct value ≤30 from SSs was the independent predictor for severe COVID-19 (HR = 10.06, 95% CI: 1.84-55.14, P = 0.008).
Conclusion: Salivary RT-qPCR testing is suitable for SARS-CoV-2 infection control, while simple measurement of Ct values can assist in prediction of COVID-19 severity.
期刊介绍：
As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies.
Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics.
Article types: original articles, notes, review articles, mini-reviews and commentaries
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
