Serum anandamide and lipids associated with linoleic acid can distinguish interstitial cystitis/bladder pain syndrome from overactive bladder: An exploratory study

IF 1.5 4区医学 Q3 UROLOGY & NEPHROLOGY

LUTS: Lower Urinary Tract Symptoms Pub Date : 2023-09-08 DOI:10.1111/luts.12501

Kazumasa Torimoto, Tomohiro Ueda, Daisuke Gotoh, Kuniyuki Kano, Makito Miyake, Yasushi Nakai, Shunta Hori, Yosuke Morizawa, Kenta Onishi, Takuto Shimizu, Mitsuru Tomizawa, Junken Aoki, Kiyohide Fujimoto

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引用次数: 0

Abstract

Objectives

Diagnosing interstitial cystitis/bladder pain syndrome presents a major challenge because it relies on subjective symptoms and empirical cystoscopic findings. A practical biomarker should discriminate diseases that cause increased urinary frequency, particularly overactive bladder. Therefore, we aimed to identify blood biomarkers that can discriminate between interstitial cystitis/bladder pain syndrome and overactive bladder.

Methods

We enrolled patients with Hunner-type interstitial cystitis (n = 20), bladder pain syndrome (n = 20), and overactive bladder (n = 20) and without lower urinary tract symptoms (controls, n = 15) at Ueda Clinic and Nara Medical University Hospital from February 2020 to August 2021. The degree of interstitial cystitis/bladder pain syndrome symptoms was evaluated using the interstitial cystitis symptom and problem indices. Metabolomics analysis was performed on 323 serum metabolites using liquid chromatography time-of-flight mass spectrometry.

Results

In the Hunner-type interstitial cystitis or bladder pain syndrome group, we observed smaller relative areas, including anandamide, acylcarnitine (18:2), linoleoyl ethanolamide, and arachidonic acid, compared to those in the overactive bladder or control group. Notably, the differences in the relative areas of anandamide were statistically significant (median: 3.950e-005 and 4.150e-005 vs. 8.300e-005 and 9.800e-005), with an area under the curve of 0.9321, demonstrating its ability to discriminate interstitial cystitis/bladder pain syndrome.

Conclusions

Serum anandamide may be a feasible diagnostic biomarker for interstitial cystitis/bladder pain syndrome. Reduced serum anandamide levels may be associated with pain and inflammation initiation, reflecting the pathology of interstitial cystitis/bladder pain syndrome. Furthermore, our findings suggest that abnormal linoleic acid metabolism may be involved in the pathogenesis of interstitial cystitis/bladder pain syndrome.

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血清anandamide和与亚油酸相关的脂质可以区分间质性膀胱炎/膀胱疼痛综合征和膀胱过度活动：一项探索性研究。

目的：诊断间质性膀胱炎/膀胱疼痛综合征是一项重大挑战，因为它依赖于主观症状和经验膀胱镜检查结果。一个实用的生物标志物应该区分导致尿频增加的疾病，尤其是膀胱过度活动。因此，我们旨在确定可以区分间质性膀胱炎/膀胱疼痛综合征和膀胱过度活动的血液生物标志物。方法：我们纳入了Hunner型间质性膀胱炎（n = 20），膀胱疼痛综合征（n = 20）和膀胱过度活动（n = 20）并且没有下尿路症状（对照组，n = 15） 2020年2月至2021年8月在上田诊所和奈良医科大学医院。间质性膀胱炎/膀胱疼痛综合征症状的程度使用间质性胱炎症状和问题指数进行评估。使用液相色谱-飞行时间质谱法对323种血清代谢物进行代谢组学分析。结果：在Hunner型间质性膀胱炎或膀胱疼痛综合征组中，与膀胱过度活动组或对照组相比，我们观察到较小的相对面积，包括anandamide、酰基肉碱（18:2）、亚麻酰乙醇酰胺和花生四烯酸。值得注意的是，阿那达明相对面积的差异具有统计学意义（中位数：3.950e-005和4.150e-005与8.300e-005和9.800e-005），曲线下面积为0.9321，表明其能够区分间质性膀胱炎/膀胱疼痛综合征。结论：血清阿那达明可能是间质性膀胱炎/膀胱疼痛综合征的一种可行的诊断生物标志物。血清anandamide水平降低可能与疼痛和炎症开始有关，反映了间质性膀胱炎/膀胱疼痛综合征的病理学。此外，我们的研究结果表明，亚油酸代谢异常可能参与间质性膀胱炎/膀胱疼痛综合征的发病机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

LUTS: Lower Urinary Tract Symptoms UROLOGY & NEPHROLOGY-

CiteScore

3.00

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： LUTS is designed for the timely communication of peer-reviewed studies which provides new clinical and basic science information to physicians and researchers in the field of neurourology, urodynamics and urogynecology. Contributions are reviewed and selected by a group of distinguished referees from around the world, some of whom constitute the journal''s Editorial Board. The journal covers both basic and clinical research on lower urinary tract dysfunctions (LUTD), such as overactive bladder (OAB), detrusor underactivity, benign prostatic hyperplasia (BPH), bladder outlet obstruction (BOO), urinary incontinence, pelvic organ prolapse (POP), painful bladder syndrome (PBS), as well as on other relevant conditions. Case reports are published only if new findings are provided. LUTS is an official journal of the Japanese Continence Society, the Korean Continence Society, and the Taiwanese Continence Society. Submission of papers from all countries are welcome. LUTS has been accepted into Science Citation Index Expanded (SCIE) with a 2011 Impact Factor.