Nanomolar range of FAM237B can activate receptor GPR83

IF 3 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Amino Acids Pub Date : 2023-09-09 DOI:10.1007/s00726-023-03328-8

Hao-Zheng Li, Ya-Fen Wang, Wen-Feng Hu, Ya-Li Liu, Zeng-Guang Xu, Zhan-Yun Guo

引用次数: 0

Abstract

Our recent study confirmed that the mature neuropeptide FAM237A, also known as neurosecretory protein GL (NPGL), is an efficient agonist for GPR83. The paralog FAM237B was previously reported as a weak agonist for GPR83. In the present study, we prepared mature human FAM237B via an intein-fusion approach and demonstrated that it could cause a significant activation effect at the nanomolar range (1‒10 nM) in a NanoBiT-based β-arrestin recruitment assay. Thus, FAM237B appears to be another endogenous agonist for GPR83 and future in vivo studies will be required to confirm this.

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FAM237B在纳摩尔范围内可激活受体GPR83。

我们最近的研究证实，成熟神经肽FAM237A，也被称为神经分泌蛋白GL (NPGL)，是GPR83的有效激动剂。类似的FAM237B先前被报道为GPR83的弱激动剂。在本研究中，我们通过注入融合方法制备了成熟的人FAM237B，并在基于nanobit的β-阻滞蛋白募集实验中证明了它可以在纳米摩尔范围(1-10 nM)内产生显著的激活效应。因此，FAM237B似乎是GPR83的另一种内源性激动剂，未来的体内研究将需要证实这一点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Amino Acids 生物-生化与分子生物学

CiteScore

6.40

自引率

5.70%

发文量

审稿时长

2.2 months

期刊介绍： Amino Acids publishes contributions from all fields of amino acid and protein research: analysis, separation, synthesis, biosynthesis, cross linking amino acids, racemization/enantiomers, modification of amino acids as phosphorylation, methylation, acetylation, glycosylation and nonenzymatic glycosylation, new roles for amino acids in physiology and pathophysiology, biology, amino acid analogues and derivatives, polyamines, radiated amino acids, peptides, stable isotopes and isotopes of amino acids. Applications in medicine, food chemistry, nutrition, gastroenterology, nephrology, neurochemistry, pharmacology, excitatory amino acids are just some of the topics covered. Fields of interest include: Biochemistry, food chemistry, nutrition, neurology, psychiatry, pharmacology, nephrology, gastroenterology, microbiology