Expression of miR-425-5p in Pancreatic Carcinoma and Its Correlation with Tumor Immune Microenvironment.

IF 2.1 4区 医学 Q2 SURGERY
Shuo Jian, Dehua Kong, Jieli Tian
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引用次数: 0

Abstract

Background: Pancreatic carcinoma (PC) is a global health threat with a high death rate. miRNAs are implicated in tumor initiation and progression. This study explored the expression of miR-425-5p in PC patients and its correlation with tumor immune microenvironment (TIME).Method: miR-425-5p expression in cancer tissues and adjacent non-tumor tissues of PC patients was examined by RT-qPCR. The levels of immune cells and cytokines were measured by flow cytometry and ELISA. The correlation of miR-425-5p with TNM stage and TIME was assessed by Spearman method. The death of PC patients was recorded through 36-month follow-ups. The prognosis of patients was assessed by Kaplan-Meier curves.Results: miR-425-5p expression was upregulated in PC tissues and elevated with increasing TNM stage. miR-425-5p expression was positively correlated with TNM stage. The PC tissues had decreased levels of CD3+, CD4+, CD8+, and natural killer (NK) cells, CD4+/CD8+ ratio, IL-2, and INF-γ, but increased levels of Tregs, IL-4, IL-10, and TGF-β. miR-425-5p level in cancer tissues was positively correlated with Tregs/IL-10/TGF-β, but negatively related to CD3+/CD4+/CD8+/NK cells and IL-2/INF-γ. Moreover, high miR-425-5p expression predicted a poor prognosis in PC patients.Conclusion: miR-425-5p is upregulated in PC patients and is prominently associated with the TIME, and high miR-425-5p predicts a poor prognosis in PC patients.

miR-425-5p在胰腺癌中的表达及其与肿瘤免疫微环境的关系
背景:胰腺癌(PC)是一种全球性的健康威胁,死亡率很高。miRNA与肿瘤的发生和发展有关。本研究探讨了miR-425-5p在PC患者中的表达及其与肿瘤免疫微环境(TIME)的相关性。方法:应用RT-qPCR方法检测PC患者癌症组织及癌旁非肿瘤组织中miR-425-5p的表达。通过流式细胞术和ELISA测定免疫细胞和细胞因子水平。通过Spearman方法评估miR-425-5p与TNM分期和TIME的相关性。通过36个月的随访记录了PC患者的死亡情况。采用Kaplan-Meier曲线评估患者的预后。结果:miR-425-5p在PC组织中的表达上调,并随着TNM分期的增加而升高。miR-425-5p的表达与TNM分期呈正相关。PC组织的CD3+、CD4+、CD8+和自然杀伤(NK)细胞水平、CD4+/CD8+比率、IL-2和INF-γ水平降低,但Tregs、IL-4、IL-10和TGF-β水平升高。癌症组织中miR-425-5p水平与Tregs/IL-10/TGF-β呈正相关,但与CD3+/CD4+/CD8+/NK细胞和IL-2/INF-γ呈负相关。此外,miR-425-5p的高表达预示着PC患者预后不良。结论:miR-425-5p在PC患者中上调,并且和TIME显著相关,并且高miR-425-5p预测PC患者的不良预后。
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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Investigative Surgery publishes peer-reviewed scientific articles for the advancement of surgery, to the ultimate benefit of patient care and rehabilitation. It is the only journal that encompasses the individual and collaborative efforts of scientists in human and veterinary medicine, dentistry, basic and applied sciences, engineering, and law and ethics. The journal is dedicated to the publication of outstanding articles of interest to the surgical research community.
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