ERK1/2 modulation in the central nervous system of male and female thiamine-deficient mice with amprolium.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Laboratory Animals Pub Date : 2024-02-01 Epub Date: 2023-09-08 DOI:10.1177/00236772231191586
Mirian P da Silva, Francisco Wb Lima, Adha Gs Moura, Julia P Nunes, Clarissa As de Cordova, Fabiano M de Cordova
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Abstract

Thiamine deficiency experimental models focus on using the pyrithiamine analog in male rodents, making the thiamine deficiency effects in females and the use of other thiamine antagonists, such as amprolium, unknown. We investigated the impact of thiamine deficiency with amprolium in the cerebral cortex and thalamus of male and female mice by evaluating the modulation of ERK1/2 phosphorylation. The animals were exposed for 20 days to thiamine-deficient chow with different doses of amprolium (20, 40, 60 and 80 mg/kg) and at different treatment periods (five, 10, 15 or 20 days) at a dose of 60 mg/kg. After treatments, ERK1/2 phosphorylation was analyzed by western blot. In male mice, we observed a progressive increase in ERK1/2 phosphorylation in both the cerebral cortex and thalamus in response to the dose of amprolium. In females, ERK1/2 phosphorylation did not progressively increase in response to the amprolium dosage. However, an increase in phosphorylation at the higher doses of 60 and 80 mg/kg was observed. We observed a more intense increase in ERK1/2 phosphorylation in males' cerebral cortex and thalamus from 10 days onwards. In females, the ERK1/2 modulation profiles were similar. The results show that thiamine deficiency induction with amprolium is efficient, compatible with other recognized models that use pyrithiamine, showing changes in cell signaling in the nervous system. The study showed differences in response to thiamine deficiency with amprolium between male and female mice in relation to ERK1/2 phosphorylation and demonstrated that females respond positively to the method and can also be used as model animals.

安普罗林对雌雄硫胺素缺乏小鼠中枢神经系统中 ERK1/2 的调节作用
硫胺素缺乏的实验模型主要是在雄性啮齿类动物中使用吡硫胺素类似物,因此雌性小鼠硫胺素缺乏的影响以及使用其他硫胺素拮抗剂(如安普罗林)的情况尚不清楚。我们通过评估ERK1/2磷酸化的调节情况,研究了雌雄小鼠大脑皮层和丘脑中使用氨溴索对硫胺素缺乏的影响。将动物暴露于硫胺素缺乏的饲料中 20 天,同时添加不同剂量的安普列林(20、40、60 和 80 毫克/千克),并在不同的处理期(5、10、15 或 20 天)添加剂量为 60 毫克/千克的安普列林。治疗后,ERK1/2 磷酸化情况通过 Western 印迹进行分析。在雄性小鼠中,我们观察到大脑皮层和丘脑中的ERK1/2磷酸化随着安络血剂量的增加而逐渐增加。在雌性小鼠中,ERK1/2磷酸化并没有随着安瓿剂量的增加而逐渐增加。然而,在 60 和 80 毫克/千克的较高剂量下,磷酸化增加。我们观察到,从10天开始,雄性大脑皮层和丘脑中的ERK1/2磷酸化增加更为剧烈。在雌性动物中,ERK1/2的调节情况类似。结果表明,使用安瓿诱导硫胺素缺乏症是有效的,与其他公认的使用吡硫胺素的模型相一致,显示了神经系统细胞信号的变化。研究表明,雄性和雌性小鼠对使用安瓿诱导硫胺素缺乏症的反应在ERK1/2磷酸化方面存在差异,并证明雌性小鼠对该方法反应积极,也可用作模型动物。
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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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