The Functionality of Membrane-Inserting Proteins and Peptides: Curvature Sensing, Generation, and Pore Formation.

IF 2.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Journal of Membrane Biology Pub Date : 2023-12-01 Epub Date: 2023-08-31 DOI:10.1007/s00232-023-00289-7
Chandra Has, Sovan Lal Das
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引用次数: 0

Abstract

Proteins and peptides with hydrophobic and amphiphilic segments are responsible for many biological functions. The sensing and generation of membrane curvature are the functions of several protein domains or motifs. While some specific membrane proteins play an essential role in controlling the curvature of distinct intracellular membranes, others participate in various cellular processes such as clathrin-mediated endocytosis, where several proteins sort themselves at the neck of the membrane bud. A few membrane-inserting proteins form nanopores that permeate selective ions and water to cross the membrane. In addition, many natural and synthetic small peptides and protein toxins disrupt the membrane by inducing nonspecific pores in the membrane. The pore formation causes cell death through the uncontrolled exchange between interior and exterior cellular contents. In this article, we discuss the insertion depth and orientation of protein/peptide helices, and their role as a sensor and inducer of membrane curvature as well as a pore former in the membrane. We anticipate that this extensive review will assist biophysicists to gain insight into curvature sensing, generation, and pore formation by membrane insertion.

Abstract Image

膜插入蛋白和多肽的功能:曲率传感、生成和孔隙形成。
具有疏水和两亲性片段的蛋白质和肽具有许多生物学功能。膜曲率的感知和产生是几个蛋白质结构域或基序的功能。虽然一些特定的膜蛋白在控制不同细胞膜的曲率方面起着重要作用,但其他的膜蛋白参与各种细胞过程,如网格蛋白介导的内吞作用,其中一些蛋白质在膜芽的颈部进行自我分类。一些插入膜的蛋白质形成纳米孔,通过选择性离子和水穿过膜。此外,许多天然和合成的小肽和蛋白毒素通过诱导膜上的非特异性孔来破坏膜。孔的形成通过内部和外部细胞内容物之间不受控制的交换导致细胞死亡。在本文中,我们讨论了蛋白质/肽螺旋的插入深度和方向,以及它们作为膜曲率的传感器和诱导剂以及膜孔形成物的作用。我们期望这一广泛的综述将有助于生物物理学家深入了解膜插入的曲率传感、产生和孔隙形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Membrane Biology
Journal of Membrane Biology 生物-生化与分子生物学
CiteScore
4.80
自引率
4.20%
发文量
63
审稿时长
6-12 weeks
期刊介绍: The Journal of Membrane Biology is dedicated to publishing high-quality science related to membrane biology, biochemistry and biophysics. In particular, we welcome work that uses modern experimental or computational methods including but not limited to those with microscopy, diffraction, NMR, computer simulations, or biochemistry aimed at membrane associated or membrane embedded proteins or model membrane systems. These methods might be applied to study topics like membrane protein structure and function, membrane mediated or controlled signaling mechanisms, cell-cell communication via gap junctions, the behavior of proteins and lipids based on monolayer or bilayer systems, or genetic and regulatory mechanisms controlling membrane function. Research articles, short communications and reviews are all welcome. We also encourage authors to consider publishing ''negative'' results where experiments or simulations were well performed, but resulted in unusual or unexpected outcomes without obvious explanations. While we welcome connections to clinical studies, submissions that are primarily clinical in nature or that fail to make connections to the basic science issues of membrane structure, chemistry and function, are not appropriate for the journal. In a similar way, studies that are primarily descriptive and narratives of assays in a clinical or population study are best published in other journals. If you are not certain, it is entirely appropriate to write to us to inquire if your study is a good fit for the journal.
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