Evaluation of the expression level of some coding and non-coding genes in oral squamous cell carcinoma

IF 2.3 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Mohadeseh Ajorlou , Parisa Bina-Jourshari , Sepideh Mirzaei , Mazaher Maghsoudloo , Mehrdad Hashemi , Neda Mousavi-Niri , Maliheh Entezari
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引用次数: 1

Abstract

Introduction

Oral squamous cell carcinoma (OSCC) is the most common cancers arising from the head and neck region. There is growing evidence that lncRNAs play an important role in OSCC progression. The study aims to investigate correlations between the expression levels of LncRNAs of PARROT, MYCNUT, DANCR, and KTN1-AS1 with clinicopathological characteristics and finding suitable biomarkers for OSCC.

Material and method

Total lncRNAs related to cancers and HNSC trascriptomics data were downloaded from lncRNADisease v2.0 database and xenabrowser, respectively. Then, ACO was perfomed on shared of LncRNAs between two databases. Finally, some lncRNAs were proposed as potential biomarkers.

Thirty biopsies samples from patients with the OSCC and 30 healthy subjects were collected by the surgery. Questionnaires including clinical and demographic data were filled for all cases. Using Real-time PCR, the expression levels of PARROT, MYCNUT, DANCR, and KTN1-AS1 lncRNAs were quantified.

Result

According to the results,17 novel gene symbol was identified.All the candidate lncRNAs the expression levels of PARROT, MYCNUT, DANCR, and KTN1-AS1 were remarkably upregulated in OSCC tumors in comparison with control group (RQ: 10.00 (P < 0.0001), RQ: 2.920 (P < 0.0001), RQ: 1.623 (P = 0.002), and 4.467 (P < 0.0001), respectively). Also, we found significant associations between tumor lncRNAs expression of PARRPT and DANCER and tumor metastasis (P = 0.009, and P = 0.005, respectively). Additionally, lncRNA KTN1-AS1 expression level was significantly higher in the patients with tumor size more than 3 cm, in comparison with tumor less than 3 cm (P = 0.005). According ROC analysis, all these candidate lncRNAs can be a significant predictor for OSCC (AUC of PARROT lncRNA = 69.72%, AUC of MYCNUT = 98.22%, AUC of DANCR = 74.83%, and AUC of KTN1-AS1 = 99.22%).

Conclusion

we found that overexpression levels of PARROT, MYCNUT, DANCR, and KTN1-AS1 lncRNAs were correlated with poor clinicopathological characteristics in patients with OSCC. Also, PARROT, MYCNUT, DANCR, and KTN1-AS1 are novel biomarker for the detection of OSCC.

口腔鳞状细胞癌中某些编码和非编码基因表达水平的评价
口腔鳞状细胞癌(OSCC)是最常见的头颈部肿瘤。越来越多的证据表明lncRNA在OSCC的进展中起着重要作用。本研究旨在研究PARROT、MYCNUT、DANCR和KTN1-AS1的LncRNA表达水平与临床病理特征之间的相关性,并寻找合适的OSCC生物标志物。材料和方法与癌症相关的总lncRNA和HNSC转录组学数据分别从lncRNADisease v2.0数据库和xenabrowser下载。然后,对两个数据库之间的LncRNA共享进行ACO。最后,提出了一些lncRNA作为潜在的生物标志物。手术收集了30名OSCC患者和30名健康受试者的活检样本。所有病例都填写了包括临床和人口统计数据在内的问卷。使用实时PCR,定量PARROT、MYCNUT、DANCR和KTN1-AS1 lncRNA的表达水平。结果共鉴定出17个新的基因符号。与对照组相比,所有候选lncRNA——PARROT、MYCNUT、DANCR和KTN1-AS1的表达水平在OSCC肿瘤中显著上调(分别为RQ:10.00(P<;0.0001)、RQ:2.920(P<)、RQ:1.623(P=0.002)和4.467(P<:0.0001))。此外,我们发现PARRPT和DANCER的肿瘤lncRNA表达与肿瘤转移之间存在显著相关性(分别为P=0.009和P=0.005)。此外,与小于3cm的肿瘤相比,肿瘤大小大于3cm的患者的lncRNA KTN1-AS1表达水平显著更高(P=0.005)。根据ROC分析,所有这些候选lncRNA都可以作为OSCC的重要预测因子(PARROT lncRNA的AUC=69.72%,MYCNUT的AUC=98.22%,DANCR的AUC=74.83%,KTN1-AS1的AUC=99.22%)。此外,PARROT、MYCNUT、DANCR和KTN1-AS1是检测OSCC的新生物标志物。
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来源期刊
Molecular and Cellular Probes
Molecular and Cellular Probes 生物-生化研究方法
CiteScore
6.80
自引率
0.00%
发文量
52
审稿时长
16 days
期刊介绍: MCP - Advancing biology through–omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.
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