SV2B defines a subpopulation of synaptic vesicles.

IF 5.3 2区 生物学 Q2 CELL BIOLOGY
Isabelle Paulussen, Hannes Beckert, Timothy F Musial, Lena J Gschossmann, Julia Wolf, Mathieu Schmitt, Jérôme Clasadonte, Georges Mairet-Coello, Christian Wolff, Susanne Schoch, Dirk Dietrich
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Abstract

Synaptic vesicles can undergo several modes of exocytosis, endocytosis, and trafficking within individual synapses, and their fates may be linked to different vesicular protein compositions. Here, we mapped the intrasynaptic distribution of the synaptic vesicle proteins SV2B and SV2A in glutamatergic synapses of the hippocampus using three-dimensional electron microscopy. SV2B was almost completely absent from docked vesicles and a distinct cluster of vesicles found near the active zone. In contrast, SV2A was found in all domains of the synapse and was slightly enriched near the active zone. SV2B and SV2A were found on the membrane in the peri-active zone, suggesting the recycling from both clusters of vesicles. SV2B knockout mice displayed an increased seizure induction threshold only in a model employing high-frequency stimulation. Our data show that glutamatergic synapses generate molecularly distinct populations of synaptic vesicles and are able to maintain them at steep spatial gradients. The almost complete absence of SV2B from vesicles at the active zone of wildtype mice may explain why SV2A has been found more important for vesicle release.

SV2B 定义了突触小泡的一个亚群。
突触小泡在单个突触内可经历多种外渗、内吞和转运模式,它们的命运可能与不同的囊泡蛋白组成有关。在这里,我们利用三维电子显微镜绘制了突触小泡蛋白 SV2B 和 SV2A 在海马谷氨酸能突触内的分布图。对接的囊泡中几乎完全没有 SV2B,在活动区附近发现了一个明显的囊泡群。相比之下,SV2A存在于突触的所有区域,并在活动区附近略有富集。SV2B和SV2A都出现在活动区周围的膜上,这表明这两簇囊泡都在循环利用。SV2B 基因敲除小鼠仅在高频刺激模型中表现出癫痫诱发阈值升高。我们的数据表明,谷氨酸能突触会产生分子上不同的突触小泡群,并能将它们维持在陡峭的空间梯度上。野生型小鼠活动区的囊泡中几乎完全没有 SV2B,这也许可以解释为什么 SV2A 被认为对囊泡释放更为重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
9.60
自引率
1.80%
发文量
1383
期刊介绍: The Journal of Molecular Cell Biology ( JMCB ) is a full open access, peer-reviewed online journal interested in inter-disciplinary studies at the cross-sections between molecular and cell biology as well as other disciplines of life sciences. The broad scope of JMCB reflects the merging of these life science disciplines such as stem cell research, signaling, genetics, epigenetics, genomics, development, immunology, cancer biology, molecular pathogenesis, neuroscience, and systems biology. The journal will publish primary research papers with findings of unusual significance and broad scientific interest. Review articles, letters and commentary on timely issues are also welcome. JMCB features an outstanding Editorial Board, which will serve as scientific advisors to the journal and provide strategic guidance for the development of the journal. By selecting only the best papers for publication, JMCB will provide a first rate publishing forum for scientists all over the world.
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