Low rate of detection of SARS-CoV-2 RNA in deceased tissue donors.

IF 1.4 4区医学 Q4 CELL BIOLOGY

Cell and Tissue Banking Pub Date : 2023-09-01 DOI:10.1007/s10561-022-10054-0

Melissa A Greenwald, Eduard Grebe, Valerie Green, Alyce Linthurst Jones, Jeffrey M Linnen, Phillip Williamson, Michael P Busch, Matthew J Kuehnert

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引用次数: 1

Abstract

Given the possibility for disease transmission, this study was performed to determine whether there is detectable SARS-CoV-2 viral RNA in the blood of deceased tissue donors. A retrospective analysis of blood samples from eligible deceased tissue donors from Oct 2019 through June 2020 was performed. Plasma aliquots were initially tested with a SARS-CoV-2 NAT Assay; positive samples were further tested using an alternate NAT and an antibody assay. The proportion of donors with confirmed RNAemia and 95% confidence intervals were computed. Of donor samples collected in 2019, 894 yielded valid results, with 6 initially positive, none of which confirmed positive by alternate NAT. Of donor samples collected in 2020, 2562 yielded valid initial NAT results, with 21 (0.8%) initially positive. Among those, 3 were confirmed by alternate NAT, 17 were not confirmed, and 1 had an invalid alternate NAT result. The rate of SARS-CoV-2 RNAemia in deceased tissue donors is approximately 1 per 1000, and it is unknown whether this RNAemia reflects the presence of infectious virus. Given these results, the risk of transmission through tissue is thought likely to be low.

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死亡组织供体中SARS-CoV-2 RNA检出率低。

考虑到疾病传播的可能性，本研究旨在确定死亡组织捐献者的血液中是否存在可检测到的SARS-CoV-2病毒RNA。对2019年10月至2020年6月符合条件的已故组织捐献者的血液样本进行了回顾性分析。最初用SARS-CoV-2 NAT Assay检测血浆等分液;阳性样本进一步使用替代NAT和抗体试验进行检测。计算确诊RNAemia的供者比例和95%置信区间。在2019年收集的供体样本中，894个产生了有效结果，其中6个初始阳性，没有一个通过替代NAT确认阳性。在2020年收集的供体样本中，2562个产生了有效的初始NAT结果，其中21个(0.8%)初始阳性。其中3个被备用NAT确认，17个未被确认，1个无效的备用NAT结果。死亡组织供体中SARS-CoV-2核糖核酸血症的发生率约为千分之一，目前尚不清楚这种核糖核酸血症是否反映了传染性病毒的存在。鉴于这些结果，通过组织传播的风险被认为可能很低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell and Tissue Banking CELL BIOLOGY-ENGINEERING, BIOMEDICAL

CiteScore

3.10

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Cell and Tissue Banking provides a forum for disseminating information to scientists and clinicians involved in the banking and transplantation of cells and tissues. Cell and Tissue Banking is an international, peer-reviewed journal that publishes original papers in the following areas: basic research concerning general aspects of tissue banking such as quality assurance and control of banked cells/tissues, effects of preservation and sterilisation methods on cells/tissues, biotechnology, etc.; clinical applications of banked cells/tissues; standards of practice in procurement, processing, storage and distribution of cells/tissues; ethical issues; medico-legal issues.