Paradoxical Expression of R-10G-reactive Antigen in Human Testicular Embryonal Carcinoma.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-10-01 Epub Date: 2023-09-07 DOI:10.1369/00221554231199134

Akifumi Muramoto, So Inamura, Hitomi Hoshino, Naoki Terada, Motohiro Kobayashi

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Abstract

Thus far, several monoclonal antibodies directed against cell-surface carbohydrate antigens have been generated. Among them, R-10G reportedly reacts selectively with human embryonic stem and induced pluripotent stem cells, but not with embryonal carcinoma (EC) cells. However, EC cells derived from patients' EC tumors may exhibit varying levels of R-10G-reactive antigen expression. Thus, we asked whether human EC tissues or germ cell tumor (GCT) tissues other than EC express R-10G-reactive antigen. To do so, we quantitatively analyzed R-10G-reactive antigen expression in 83 testicular GCT surgical specimens containing a total of 125 various GCT components. Accordingly, in all EC components examined, the EC cell plasma membrane was immunolabeled with R-10G, while most seminoma components were R-10G-negative. In non-seminomatous GCT (NSGCT) other than EC (non-EC NSGCT), R-10G-reactive antigen expression was variable, but signal distribution was focal, and the average intensity was weaker than that seen in EC. The percentages of R-10G-positive cells in these three groups varied with high statistical significance (p<0.001 for all combinations). These findings indicate that the R-10G-reactive antigen is preferentially expressed in human testicular EC tissues and, thus, could be used as a diagnostic marker for this malignancy.

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R-10G反应性抗原在人睾丸胚胎癌中的异常表达。

到目前为止，已经产生了几种针对细胞表面碳水化合物抗原的单克隆抗体。其中，据报道，R-10G选择性地与人类胚胎干细胞和诱导多能干细胞反应，但不与胚胎癌（EC）细胞反应。然而，来源于患者EC肿瘤的EC细胞可能表现出不同水平的R-10G反应性抗原表达。因此，我们询问人类EC组织或EC以外的生殖细胞肿瘤（GCT）组织是否表达R-10G反应性抗原。为此，我们定量分析了83个睾丸GCT手术标本中R-10G反应性抗原的表达，这些标本共含有125种不同的GCT成分。因此，在所有检查的EC成分中，EC细胞质膜用R-10G免疫标记，而大多数精原细胞瘤成分为R-10G阴性。在EC以外的非精原细胞性GCT（NSGCT）中，R-10G反应性抗原的表达是可变的，但信号分布是局灶性的，平均强度比EC弱

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.