Meiotic segregation and post-meiotic drive of the Festuca pratensis B chromosome.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chromosome Research Pub Date : 2023-09-02 DOI:10.1007/s10577-023-09728-6

Rahman Ebrahimzadegan, Jörg Fuchs, Jianyong Chen, Veit Schubert, Armin Meister, Andreas Houben, Ghader Mirzaghaderi

{"title":"Meiotic segregation and post-meiotic drive of the Festuca pratensis B chromosome.","authors":"Rahman Ebrahimzadegan, Jörg Fuchs, Jianyong Chen, Veit Schubert, Armin Meister, Andreas Houben, Ghader Mirzaghaderi","doi":"10.1007/s10577-023-09728-6","DOIUrl":null,"url":null,"abstract":"<p><p>In many species, the transmission of B chromosomes (Bs) does not follow the Mendelian laws of equal segregation and independent assortment. This deviation results in transmission rates of Bs higher than 0.5, a process known as \"chromosome drive\". Here, we studied the behavior of the 103 Mbp-large B chromosome of Festuca pratensis during all meiotic and mitotic stages of microsporogenesis. Mostly, the B chromosome of F. pratensis segregates during meiosis like standard A chromosomes (As). In some cases, the B passes through meiosis in a non-Mendelian segregation leading to their accumulation already in meiosis. However, a true drive of the B happens during the first pollen mitosis, by which the B preferentially migrates to the generative nucleus. During second pollen mitosis, B divides equally between the two sperms. Despite some differences in the frequency of drive between individuals with different numbers of Bs, at least 82% of drive was observed. Flow cytometry-based quantification of B-containing sperm nuclei agrees with the FISH data.</p>","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"31 3","pages":"26"},"PeriodicalIF":2.4000,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474989/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromosome Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10577-023-09728-6","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

In many species, the transmission of B chromosomes (Bs) does not follow the Mendelian laws of equal segregation and independent assortment. This deviation results in transmission rates of Bs higher than 0.5, a process known as "chromosome drive". Here, we studied the behavior of the 103 Mbp-large B chromosome of Festuca pratensis during all meiotic and mitotic stages of microsporogenesis. Mostly, the B chromosome of F. pratensis segregates during meiosis like standard A chromosomes (As). In some cases, the B passes through meiosis in a non-Mendelian segregation leading to their accumulation already in meiosis. However, a true drive of the B happens during the first pollen mitosis, by which the B preferentially migrates to the generative nucleus. During second pollen mitosis, B divides equally between the two sperms. Despite some differences in the frequency of drive between individuals with different numbers of Bs, at least 82% of drive was observed. Flow cytometry-based quantification of B-containing sperm nuclei agrees with the FISH data.

Abstract Image

查看原文本刊更多论文

高羊茅B染色体的减数分裂和减数分裂后驱动。

在许多物种中，B染色体（Bs）的传播不遵循孟德尔的平等分离和独立分类定律。这种偏差导致Bs的传播率高于0.5，这一过程被称为“染色体驱动”。在这里，我们研究了高羊茅103Mbp大B染色体在小孢子发生的所有减数分裂和有丝分裂阶段的行为。大多数情况下，pratensis的B染色体在减数分裂过程中像标准的A染色体（As）一样分离。在某些情况下，B以非孟德尔分离的方式通过减数分裂，导致它们已经在减数分裂中积累。然而，B的真正驱动作用发生在第一次花粉有丝分裂期间，通过这种有丝分裂，B优先迁移到生殖细胞核。在第二次花粉有丝分裂过程中，B在两个精子之间平分。尽管具有不同B数的个体之间的驱动频率存在一些差异，但至少观察到82%的驱动。基于流式细胞术的含B精子细胞核定量与FISH数据一致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chromosome Research 生物-生化与分子生物学

CiteScore

4.70

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Chromosome Research publishes manuscripts from work based on all organisms and encourages submissions in the following areas including, but not limited, to: · Chromosomes and their linkage to diseases; · Chromosome organization within the nucleus; · Chromatin biology (transcription, non-coding RNA, etc); · Chromosome structure, function and mechanics; · Chromosome and DNA repair; · Epigenetic chromosomal functions (centromeres, telomeres, replication, imprinting, dosage compensation, sex determination, chromosome remodeling); · Architectural/epigenomic organization of the genome; · Functional annotation of the genome; · Functional and comparative genomics in plants and animals; · Karyology studies that help resolve difficult taxonomic problems or that provide clues to fundamental mechanisms of genome and karyotype evolution in plants and animals; · Mitosis and Meiosis; · Cancer cytogenomics.