L-serine combined with carboxymethyl chitosan guides amorphous calcium phosphate to remineralize enamel

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2023-09-02 DOI:10.1007/s10856-023-06745-z

Yinghui Wang, Shuting Zhang, Peiwen Liu, Fan Li, Xu Chen, Haorong Wang, Zhangyi Li, Xi Zhang, Xiangyu Zhang, Xu Zhang

{"title":"L-serine combined with carboxymethyl chitosan guides amorphous calcium phosphate to remineralize enamel","authors":"Yinghui Wang, Shuting Zhang, Peiwen Liu, Fan Li, Xu Chen, Haorong Wang, Zhangyi Li, Xi Zhang, Xiangyu Zhang, Xu Zhang","doi":"10.1007/s10856-023-06745-z","DOIUrl":null,"url":null,"abstract":"<div><p>The aim of this study is to investigate a robust and stable calcium-phosphorus system to remineralize human early enamel caries lesions with nanocomplexes of carboxymethyl chitosan/L-serine/amorphous calcium phosphate (CMC-Ser-ACP) to develop an effective method for mimicking the amelogenin (AMEL) mineralization pattern through ACP assembly. A CMC-Ser-ACP nanocomplex solution was first synthesized by a chemical precipitation method, and then 1% sodium hypochlorite (NaClO) was added to induce ACP phase formation. The morphologies of the nanocomplexes were characterized by transmission electron microscopy (TEM), and zeta potential analysis and Fourier transform infrared spectroscopy (FTIR) were performed to detect surface charge and functional group changes. The subtle changes of the demineralized enamel models induced by the remineralization effect were observed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The CMC-Ser-ACP nanocomplex solution could be preserved without any precipitation for 45 days. After the application of NaClO and through the guidance of Ser, ACP nanoparticles transformed into relatively orderly arranged hydroxyapatite (HAP) crystals, generating an aprismatic enamel-like layer closely integrated with the demineralized enamel, which resulted in enhanced mechanical properties for the treatment of early enamel caries lesions. The CMC-Ser-ACP nanocomplex solution is a remineralization system with great solution stability, and when NaClO is added, it can rapidly regenerate an aprismatic enamel-like layer in situ on the demineralized enamel surface. This novel remineralization system has stable chemical properties and can greatly increase the therapeutic effects against early enamel caries.</p><h3>Graphical Abstract</h3>\n <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\n </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 9","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474979/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Materials Science: Materials in Medicine","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10856-023-06745-z","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 0

Abstract

The aim of this study is to investigate a robust and stable calcium-phosphorus system to remineralize human early enamel caries lesions with nanocomplexes of carboxymethyl chitosan/L-serine/amorphous calcium phosphate (CMC-Ser-ACP) to develop an effective method for mimicking the amelogenin (AMEL) mineralization pattern through ACP assembly. A CMC-Ser-ACP nanocomplex solution was first synthesized by a chemical precipitation method, and then 1% sodium hypochlorite (NaClO) was added to induce ACP phase formation. The morphologies of the nanocomplexes were characterized by transmission electron microscopy (TEM), and zeta potential analysis and Fourier transform infrared spectroscopy (FTIR) were performed to detect surface charge and functional group changes. The subtle changes of the demineralized enamel models induced by the remineralization effect were observed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The CMC-Ser-ACP nanocomplex solution could be preserved without any precipitation for 45 days. After the application of NaClO and through the guidance of Ser, ACP nanoparticles transformed into relatively orderly arranged hydroxyapatite (HAP) crystals, generating an aprismatic enamel-like layer closely integrated with the demineralized enamel, which resulted in enhanced mechanical properties for the treatment of early enamel caries lesions. The CMC-Ser-ACP nanocomplex solution is a remineralization system with great solution stability, and when NaClO is added, it can rapidly regenerate an aprismatic enamel-like layer in situ on the demineralized enamel surface. This novel remineralization system has stable chemical properties and can greatly increase the therapeutic effects against early enamel caries.

Graphical Abstract

Abstract Image

查看原文本刊更多论文

L-丝氨酸与羧甲基壳聚糖结合引导无定形磷酸钙对牙釉质进行再矿化。

本研究的目的是用羧甲基壳聚糖/L-丝氨酸/无定形磷酸钙纳米复合物（CMC Ser-ACP）研究一种坚固稳定的钙磷系统来再矿化人类早期釉质龋损伤，以开发一种通过ACP组装模拟釉原蛋白（AMEL）矿化模式的有效方法。首先通过化学沉淀法合成了CMC-Ser-ACP纳米复合物溶液，然后加入1%的次氯酸钠（NaClO）以诱导ACP相的形成。通过透射电子显微镜（TEM）对纳米复合物的形貌进行了表征，并进行了ζ电位分析和傅里叶变换红外光谱（FTIR）来检测表面电荷和官能团的变化。通过扫描电子显微镜（SEM）和X射线衍射（XRD）观察了再矿化效应引起的脱矿釉质模型的细微变化。CMC Ser-ACP纳米复合物溶液可以在没有任何沉淀的情况下保存45天。应用NaClO后，在Ser的引导下，ACP纳米颗粒转化为排列相对有序的羟基磷灰石（HAP）晶体，产生与脱矿釉质紧密结合的杏仁状釉质层，从而提高了治疗早期釉质龋损伤的力学性能。CMC-Ser-ACP纳米复合物溶液是一种具有良好溶液稳定性的再矿化系统，当加入NaClO时，它可以在脱矿牙釉质表面原位快速再生一层杏仁状牙釉质层。这种新型的再矿化系统具有稳定的化学性质，可以大大提高对早期釉质龋的治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.