Eva Grabner , Eva Stare , Lijana Fanedl , Maša Zorec , Dakota S. Jones , Christopher D. Johnston , Gorazd Avguštin , Tomaž Accetto
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引用次数: 0
Abstract
27 strains representing eight new Prevotella species were isolated from rumen of a single sheep in eight weeks interval. One of the putative species encompassing the highest number of isolated strains which also exhibited some genetic variability in preliminary data, was then selected for description of a novel species. We examined six strains in genomic and phenotypic detail, two of which may actually be the same strain isolated nearly three weeks apart. Other strains formed clearly diverged intraspecies lineages as evidenced by core genome phylogeny and phenotypic differences. Strains of the proposed new Prevotella species are strictly saccharolytic as is usual for rumen Prevotella, and use plant cell-wall xylans and pectins for growth. However, the range of cell-wall polysaccharides utilised for growth is rather limited compared to rumen generalists such as Prevotella bryantii or Prevotella ruminicola and this extends also to the inability to utilise starch, which is unexpected for the members of the genus Prevotella. Based on the data obtained, we propose Prevotella communis sp. nov. to accommodate strain E1-9T as well as other strains with the similar properties. The proposed species is widespread: two other strains were previously isolated from sheep in Japan and is also common in metagenomic data of cattle and sheep rumen samples from Scotland and New Zealand. It was also found in a collection of metagenome-assembled genomes originating from cattle in Scotland. Thus, it is a ubiquitous bacterium of domesticated ruminants specialising in degradation of a somewhat restricted set of plant cell wall components.
在8周的时间间隔内从一只羊的瘤胃中分离出8个新的普雷沃氏菌种27株。其中一个假定的物种包含最高数量的分离菌株,并且在初步数据中也表现出一些遗传变异,然后被选择用于描述一个新种。我们在基因组和表型细节上检查了六株菌株,其中两株实际上可能是相隔近三周分离的同一株菌株。其他菌株形成了明显分化的种内谱系,核心基因组系统发育和表型差异证明了这一点。与通常的瘤胃普雷沃特菌一样,提出的新普雷沃特菌株严格地具有糖溶性,并利用植物细胞壁木聚糖和果胶进行生长。然而,用于生长的细胞壁多糖的范围与瘤胃通才(如bryantii普雷沃氏菌或ruminicola普雷沃氏菌)相比相当有限,这也延伸到无法利用淀粉,这对普雷沃氏菌属的成员来说是意想不到的。根据获得的数据,我们提出了普雷沃氏菌(Prevotella communis sp. 11 .)来容纳菌株E1-9T以及其他具有类似性质的菌株。该物种分布广泛:之前从日本的绵羊中分离出另外两种菌株,在苏格兰和新西兰的牛和羊瘤胃样本的元基因组数据中也很常见。它也被发现在一组来自苏格兰牛的宏基因组组装基因组中。因此,它是一种在驯养反刍动物中普遍存在的细菌,专门降解某种程度上有限的植物细胞壁成分。
期刊介绍:
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: