The effects of cisplatin and jaceosidin on SH-SY5Y neuroblastoma cells: an electron microscopic, molecular and biochemical study.

IF 1.1 4区医学 Q4 MICROSCOPY

Ultrastructural Pathology Pub Date : 2023-09-03 DOI:10.1080/01913123.2023.2218911

Pinar Bayram, Selina Aksak Karamese, Bengul Özdemir, Aysegul Durak, Deniz Billur

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引用次数: 0

Abstract

In this study, our aim was to show both the single and combined effects of cisplatin and jaceosidin in SHSY-5Y neuroblastoma cells. For this purpose, we used MTT cellular viability assay, Enzyme-Linked Immunosorbent Assay (ELISA), Transmission Electron Microscopy (TEM), Immunofluorescence Staining Assay (IFA) and Western blotting (WB) assay. According to MTT findings, IC50 dose was detected as 50 µM cisplatin and 160 µM jaceosidin co-application. Therefore, experimental groups were finally selected as control, cisplatin, 160 µM jaceosidin and Cisplatin +160 µM jaceosidin. Cell viability was decreased in all groups, and the IFA findings confirmed the viability analysis. WB data indicated that matrix metalloproteinase 2 and 9 levels, as indicators of metastasis, decreased. While LPO and CAT levels increased in all treatment groups, it was observed that the activity of SOD decreased. When TEM micrographs were investigated, cellular damages were determined. In the light of these results, it can be said that cisplatin and jaceosidin have a potential to increase the effects of each other synergistically.

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顺铂和紫叶黄素对SH-SY5Y神经母细胞瘤细胞的影响:电镜、分子和生化研究。

在这项研究中，我们的目的是显示顺铂和jaceosidin在SHSY-5Y神经母细胞瘤细胞中的单独和联合作用。为此，我们使用了MTT细胞活力测定、酶联免疫吸附测定(ELISA)、透射电镜(TEM)、免疫荧光染色测定(IFA)和Western blotting (WB)测定。根据MTT结果，检测IC50剂量为50µM顺铂与160µM紫皮苷联合应用。因此，最终选择试验组为对照组，顺铂+160µM jacosidin和顺铂+160µM jacosidin。各组细胞活力均下降，IFA结果证实了细胞活力分析。WB数据显示，作为转移指标的基质金属蛋白酶2和9水平下降。各处理组LPO和CAT水平升高，SOD活性降低。当TEM显微图被调查时，细胞损伤被确定。鉴于这些结果，可以说顺铂和jaceosidin具有协同增加彼此作用的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ultrastructural Pathology 医学-病理学

CiteScore

2.00

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.