Optimal Extraction and Deproteinization Method for Mannoprotein Purification from Kluyveromyces marxianus.

Q2 Biochemistry, Genetics and Molecular Biology

Iranian Biomedical Journal Pub Date : 2023-09-01 Epub Date: 2023-02-12 DOI:10.61186/ibj.27.5.320

Ashraf Hajhosseini, Anousheh Sharifan, Zohreh Eftekhari, Ariana Alavi, Delaram Doroud

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引用次数: 1

Abstract

Background: Mannoproteins, mannose-glycosylated proteins, play an important role in biological processes and have various applications in industries. Several methods have been already used for the extraction of mannoproteins from yeast cell-wall. The aim of this study was to evaluate the extraction and deproteinization of mannan oligosaccharide from the Kluyveromyces (K.) marxianus mannoprotein.

Methods: To acquire crude mannan oligosaccharides, K. marxianus mannoproteins were deproteinized by the Sevage, trichloroacetic acid, and hydrochloric acid (HCL) methods. Total nitrogen, crude protein content, fat, carbohydrate and ash content were measured according to the monograph prepared by the meeting of the Joint FAO/WHO Expert Committee and standard. Mannan oligosaccharide loss, percentage of deproteinization, and chemical composition of the product were assessed to check the proficiency of different methods.

Results: Highly purified (95.4%) mannan oligosaccharide with the highest deproteinization (97.33 ± 0.4%) and mannan oligosaccharide loss (25.1 ± 0.6%) were obtained following HCl method.

Conclusion: HCl, was the most appropriate deproteinization method for the removal of impurities. This preliminary data will support future studies to design scale-up procedures.

查看原文本刊更多论文

马氏克鲁维菌甘露蛋白的最佳提取及脱蛋白方法。

背景:甘露糖基化蛋白在生物过程中起着重要作用，在工业上有着广泛的应用。从酵母细胞壁中提取甘露蛋白已有几种方法。本研究的目的是评价从马氏克鲁维酵母甘露蛋白中提取和脱蛋白甘露聚糖的工艺。方法:采用Sevage法、三氯乙酸法和盐酸(HCL)法对马氏甘露聚糖蛋白进行脱蛋白处理，得到甘露聚糖粗多糖。总氮、粗蛋白质、脂肪、碳水化合物和灰分含量根据FAO/WHO联合专家委员会会议编制的专著和标准进行测定。对甘露聚糖的损失、脱蛋白率和产品的化学成分进行了评估，以检验不同方法的熟练程度。结果:高纯度甘露聚糖(95.4%)，脱蛋白率最高(97.33±0.4%)，低聚糖损失率最高(25.1±0.6%)。结论:盐酸是去除杂质最合适的脱蛋白方法。这些初步数据将支持未来的研究，以设计扩大规模的程序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Biomedical Journal Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.20

自引率

0.00%

发文量

审稿时长

8 weeks