E2F8 knockdown suppresses cell proliferation and induces cell cycle arrest via Wnt/β-Catenin pathway in ovarian cancer.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-07-01 DOI:10.4103/cjop.CJOP-D-22-00142

Meiyin Zhang, Ye Xu, Yongjian Zhang, Ge Lou

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引用次数: 1

Abstract

Ovarian cancer is one of the leading causes of death in female reproductive system cancers. However, the pathogenesis of ovarian cancer remains elusive. Our aim is to investigate the potential targets for ovarian cancer. Two microarray datasets were obtained from the Gene Expression Omnibus public database. Using R package limma, the differentially expressed genes (DEGs) were identified from the datasets. There were 95 overlapping DEGs in two microarray datasets. GO, KEGG pathway analysis, and protein-protein interaction (PPI) network analysis were carried out based on the DEGs. Wnt signaling pathway and cell cycle were enriched in the KEGG pathway analysis. Moreover, the top 10 hub genes with the most nodes were determined by PPI network analysis. E2F8, one of hub genes was positively linked to a bad outcome in ovarian cancer patients. Furthermore, E2F8 knockdown suppressed cell proliferation and induced cell cycle arrest in ovarian cancer. In addition, we found that silencing E2F8 inhibited the Wnt/β-catenin signaling pathway. In ovarian cancer cells with E2F8 knockdown, overexpressing β-catenin restored both the suppressed capacity of cell proliferation and cell cycle progression. Therefore, our results revealed that E2F8 had an involvement in the development of ovarian cancer which might act as a therapeutic target.

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E2F8敲低抑制卵巢癌症细胞增殖，并通过Wnt/β-儿茶素途径诱导细胞周期阻滞。

癌症是女性生殖系统癌症死亡的主要原因之一。然而，癌症的发病机制仍然难以捉摸。我们的目的是研究卵巢癌症的潜在靶点。从基因表达综合公共数据库中获得两个微阵列数据集。使用R软件包limma，从数据集中鉴定差异表达基因（DEGs）。在两个微阵列数据集中有95个重叠的DEG。基于DEG进行GO、KEGG通路分析和蛋白质-蛋白质相互作用（PPI）网络分析。在KEGG通路分析中富集了Wnt信号通路和细胞周期。此外，通过PPI网络分析确定了具有最多节点的前10个枢纽基因。E2F8，中枢基因之一与卵巢癌症患者的不良结局呈正相关。此外，E2F8敲除抑制了卵巢癌症的细胞增殖并诱导细胞周期阻滞。此外，我们发现沉默E2F8抑制Wnt/β-catenin信号通路。在E2F8敲低的癌症卵巢细胞中，过表达β-连环蛋白恢复了细胞增殖和细胞周期进程的抑制能力。因此，我们的研究结果表明，E2F8参与了卵巢癌症的发展，这可能是一个治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.