Effect assessment of a type of xeno-free and serum-free human adipose-derived mesenchymal stem cells culture medium by proliferation and differentiation capacities.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Cytotechnology Pub Date : 2023-10-01 Epub Date: 2023-07-11 DOI:10.1007/s10616-023-00586-y
Shanshan Chen, Li Meng, Shanshan Wang, Yan Xu, Wenbin Chen, Jianfeng Wei
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引用次数: 0

Abstract

Human mesenchymal stem cells (hMSCs) possess broad prospects in pre-clinical research. In vitro amplification of hMSCs requires appropriate medium to reach the number of seed cells with clinical significance. However, the uncertainty of the heterologous components of the traditional fetal bovine serum (FBS) culture medium has great safety risks. Moreover, existing commercial hMSCs medium is very expensive, therefore a safer and more optimal hMSCs medium is urgently needed. Accordingly, we developed five components adipose-derived hMSCs (hADMSCs) medium without xenogenic components, named E5 SFM. which is mainly composed of knockout serum replacement (KSR), and additionally four components such as fibroblast growth factor and transferrin. Here, we mainly compared the E5 SFM with traditional FBS-containing medium and a commercial medium by surface markers testing, proliferation assay as well as osteogenic, adipogenic and chondrogenic differentiation assessment. We demonstrated that hADMSCs cultured in the E5 SFM showed similar morphological characteristics and immunophenotypes to those in other media. Notably, cell proliferative capability was similar to that in the commercial medium, but higher than that in the FBS-containing medium and other media. Additionally, their capabilities of adipogenic and osteogenic differentiation were significantly higher than those of other media. Consequently, we concluded that the E5 SFM medium can not only effectively promote cell proliferation of hMSCs, but also has optimal differentiative capacity and clear and simple ingredients.

Abstract Image

通过增殖和分化能力评估一种无异种和无血清的人脂肪来源的间充质干细胞培养基的效果。
人间充质干细胞在临床前研究中具有广阔的前景。hMSCs的体外扩增需要合适的培养基才能达到具有临床意义的种子细胞数量。然而,传统胎牛血清(FBS)培养基异源成分的不确定性存在很大的安全风险。此外,现有的商业化hMSCs培养基非常昂贵,因此迫切需要一种更安全、更优化的hMSCs介质。因此,我们开发了不含异种成分的五组分脂肪来源的hMSCs(hADMSCs)培养基,命名为E5-SFM。其主要由敲除血清置换(KSR)和另外四种成分如成纤维细胞生长因子和转铁蛋白组成。在这里,我们主要通过表面标记物测试、增殖测定以及成骨、成脂和软骨分化评估,将E5 SFM与传统的含FBS的培养基和商业培养基进行比较。我们证明,在E5 SFM中培养的hADMSC表现出与其他培养基相似的形态学特征和免疫表型。值得注意的是,细胞增殖能力与商业培养基相似,但高于含FBS的培养基和其他培养基。此外,它们的成脂和成骨分化能力显著高于其他培养基。因此,我们得出结论,E5 SFM培养基不仅可以有效地促进hMSCs的细胞增殖,而且具有最佳的分化能力和清晰简单的成分。
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来源期刊
Cytotechnology
Cytotechnology 生物-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
49
审稿时长
6-12 weeks
期刊介绍: The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.
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