Effect of milk and whey on proliferation and differentiation of placental stromal cells.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Cytotechnology Pub Date : 2023-10-01 Epub Date: 2023-07-17 DOI:10.1007/s10616-023-00585-z
Bircan Boga, Merve Akbulut, Erkan Maytalman, Ilknur Kozanoglu
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引用次数: 0

Abstract

Fetal bovine serum (FBS), which is widely used in cell culture media, has the potential to cause medical and ethical problems. Here, an experimental study using milk or whey proteins containing essential nutrients and growth factors is presented to limit the use of FBS in cell culture media produced for cell and tissue regeneration. Study groups were formed by culturing human placenta mesenchymal stem cells, known to have high proliferation and differentiation capacity, with milk or whey solution at increasing concentrations, alone or in combination with FBS. Osteogenic and adipogenic differentiation capacities of proliferating cells were observed in FBS, milk or whey groups. Milk, whey or FBS groups obtained in P3 and after differentiation were separately analyzed for protein mRNA expression by reverse transcriptase-polymerase chain reaction (RT-qPCR). Fibroblast Growth Factor 2 (FGF2), Octamer-binding Transcription Factor 4 (OCT4), Bone Morphogenetic Protein 6 (BMP6), and adipogenic differentiation marker Peroxisome Proliferator-Activated Receptor Gamma (PPARG) were analysed by RT-qPCR. Proliferation was more pronounced in FBS alone and in its combinations with milk-whey compared to the groups in which only milk and whey were used. OCT4 mRNA and FGF2 mRNA expression decreased in differentiated cells. BMP6 mRNA expression increased with osteogenic and adipogenic stimuli. As expected, PPRG expression also increased with adipogenic stimulation. With this experimental study, evidence has been obtained that milk or whey can provide nutritional support to the culture media of repair cells and preserve the functional capacity of the cells, with a slightly more limited capacity than FBS.

Graphical abstract:

Supplementary information: The online version contains supplementary material available at 10.1007/s10616-023-00585-z.

Abstract Image

牛奶和乳清对胎盘基质细胞增殖和分化的影响。
胎牛血清广泛应用于细胞培养基中,有可能引发医学和伦理问题。在这里,一项使用含有必需营养素和生长因子的牛奶或乳清蛋白的实验研究旨在限制FBS在为细胞和组织再生生产的细胞培养基中的使用。研究组是通过将已知具有高增殖和分化能力的人胎盘间充质干细胞与浓度不断增加的牛奶或乳清溶液单独或与FBS联合培养而形成的。在FBS、牛奶或乳清组中观察到增殖细胞的成骨和成脂分化能力。通过逆转录聚合酶链式反应(RT-qPCR)分别分析在P3和分化后获得的牛奶、乳清或FBS组的蛋白质mRNA表达。通过RT-qPCR分析成纤维细胞生长因子2(FGF2)、八聚体结合转录因子4(OCT4)、骨形态发生蛋白6(BMP6)和脂肪分化标记物过氧化物酶体增殖因子激活受体γ(PPARG)。与仅使用牛奶和乳清的组相比,单独使用FBS及其与牛奶乳清的组合中的增殖更明显。OCT4 mRNA和FGF2 mRNA在分化细胞中的表达降低。BMP6 mRNA表达随着成骨和成脂刺激而增加。正如预期的那样,PPRG的表达也随着脂肪生成刺激而增加。通过这项实验研究,已经获得证据表明,牛奶或乳清可以为修复细胞的培养基提供营养支持,并保持细胞的功能能力,其能力略高于FBS。图形摘要:补充信息:在线版本包含补充材料,可访问10.1007/s10616-023-00585-z。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cytotechnology
Cytotechnology 生物-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
49
审稿时长
6-12 weeks
期刊介绍: The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.
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