Applications of Environmental DNA (eDNA) in Monitoring the Endangered Status and Evaluating the Stock Enhancement Effect of Tropical Sea Cucumber Holothuria Scabra

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Marine Biotechnology Pub Date : 2023-09-02 DOI:10.1007/s10126-023-10239-y

Zixuan E, Peng Luo, Chunhua Ren, Chuhang Cheng, Wenjie Pan, Xiao Jiang, Fajun Jiang, Bo Ma, Suzhong Yu, Xin Zhang, Ting Chen, Chaoqun Hu

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Abstract

The tropical sea cucumber Holothuria scabra is naturally found in the Indo-West Pacific. However, due to their commercial value, natural H. scabra populations have declined significantly in recent years, resulting in its status as an endangered species. Surveys of H. scabra resource pose a challenge due to its specific characteristics, such as sand-burrowing behavior. To overcome this problem, our study established a convenient and feasible method for assessing H. scabra resources using environmental DNA (eDNA) monitoring technology. First, H. scabra-specific TaqMan primers and probe were designed based on its cox1 gene, followed by the development of an eDNA monitoring method for H. scabra in two separate sea areas (Xuwen and Daya Bay). The method was subsequently employed to investigate the distribution of H. scabra and assess the effects of aquaculture stock enhancement through juvenile releasing in the Weizhou Island sea area. The H. scabra eDNA monitoring approach was found to be more appropriate and credible than traditional methods, and a positive impact of stocking on H. scabra populations was observed. In summary, this is the first report to quantify eDNA concentration in a Holothuroidea species, and it provides a convenient and accurate method for surveying H. scabra resources. This study introduces novel concepts for eDNA-based detection of endangered marine benthic animals and monitoring their population distribution and abundance.

Graphical Abstract

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环境DNA (eDNA)在热带海参(Holothuria Scabra)濒危状况监测及种群增养效果评价中的应用

热带海参Holothuria scabra天然存在于印度-西太平洋。然而，由于其商业价值，近年来自然种群数量明显下降，导致其成为濒危物种。由于其独特的特征，如钻沙行为，对其资源调查提出了挑战。为了克服这一问题，本研究建立了一种方便可行的利用环境DNA (environmental DNA, eDNA)监测技术评价黄颡鱼资源的方法。首先，基于其cox1基因，设计了黄鳞蟹特异性TaqMan引物和探针，然后在徐文和大亚湾两个不同海域建立了黄鳞蟹eDNA监测方法。利用该方法调查了涠洲岛海域黄颡鱼的分布情况，并对放养幼鱼增加养殖种群的效果进行了评价。结果表明，与传统的监测方法相比，该方法更适宜、更可信，放养对黄颡鱼种群有积极影响。综上所述，本研究首次定量报道了黄颡鱼科物种的eDNA浓度，为黄颡鱼资源调查提供了一种方便、准确的方法。本研究介绍了基于edna的濒危海洋底栖动物检测及其种群分布和丰度监测的新概念。

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来源期刊

Marine Biotechnology 工程技术-海洋与淡水生物学

CiteScore

4.80

自引率

3.30%

发文量

审稿时长

2 months

期刊介绍： Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.