Tuft Cells Are Present in Submandibular Glands Across Species.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-09-01 Epub Date: 2022-08-21 DOI:10.1369/00221554221120301

Harim Tavares Dos Santos, Kihoon Nam, Frank M Maslow, Travis Small, Tabitha L I Galloway, Laura M Dooley, Patrick T Tassone, Robert P Zitsch, Gary A Weisman, Olga J Baker

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Abstract

Tuft cells are bottle-shaped, microvilli-projecting chemosensory cells located in the lining of a variety of epithelial tissues and, following their identification approximately 60 years ago, have been linked to immune system function in a variety of epithelia. Until recently, Tuft cells had not been convincingly demonstrated to be present in salivary glands with their detection by transmission electron microscopy only shown in a handful of earlier studies using rat salivary glands, and no follow-up work has been conducted to verify their presence in salivary glands of other species. Here, we demonstrate that Tuft cells are present in the submandibular glands of various species (i.e., mouse, pig and human) using transmission electron microscopy and confocal immunofluorescent analysis for the POU class 2 homeobox 3 (POU2F3), which is considered to be a master regulator of Tuft cell identity.

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不同物种的下颌下腺中都存在簇细胞

Tuft细胞是一种瓶形的微绒毛突起化感细胞，位于多种上皮组织的内壁，大约60年前被发现后，就被认为与多种上皮组织的免疫系统功能有关。直到最近，Tuft 细胞还没有被令人信服地证明存在于唾液腺中，仅在少数使用大鼠唾液腺的早期研究中通过透射电子显微镜发现了它们，而且还没有后续工作来验证它们是否存在于其他物种的唾液腺中。在这里，我们利用透射电子显微镜和共聚焦免疫荧光分析 POU 2 类同源染色体 3（POU2F3），证明 Tuft 细胞存在于不同物种（如小鼠、猪和人类）的颌下腺中，而 POU 类同源染色体 3 被认为是 Tuft 细胞特征的主调控因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.