Evaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancer.

IF 3.1 2区医学 Q3 IMMUNOLOGY

Infectious Agents and Cancer Pub Date : 2023-08-29 DOI:10.1186/s13027-023-00523-w

Leila Dadgar-Zankbar, Aref Shariati, Narjess Bostanghadiri, Zahra Elahi, Shiva Mirkalantari, Shabnam Razavi, Fatemeh Kamali, Davood Darban-Sarokhalil

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引用次数: 1

Abstract

Background: Colorectal cancer (CRC) is one of the most common cancers all over the world, and dysbiosis in the gut microbiota may play a role in colorectal carcinogenesis. Bacteroides fragilis can lead to tumorigenesis by changing signaling pathways, including the WNT/β-catenin pathway. Therefore, in the present study, we investigated the correlation between the enterotoxigenic B. fragilis amount and the expression of signaling pathway genes involved in CRC.

Materials and methods: B. fragilis was determined in 30 tumors and adjacent healthy tissues by the qPCR method. Next, the relationship between enterotoxigenic B. fragilis and the expression of signaling pathway genes, including CCND1, TP53, BCL2, BAX, WNT, TCF, AXIN, APC, and CTNNB1 was investigated. Additionally, possible correlations between clinicopathological features of the tumor samples and the abundance of B. fragilis were analyzed.

Results: The results showed that B. fragilis was detected in 100% of tumor samples and 86% of healthy tissues. Additionally, enterotoxigenic B. fragilis colonized 47% of all samples, and bft-1 toxin was the most frequently found isotype among the samples. The analysis showed that the high level of B. fragilis has a significant relationship with the high expression of AXIN, CTNNB1, and BCL2 genes. On the other hand, our results did not show any possible correlation between this bacterium and the clinicopathological features of the tumor sample.

Conclusion: B. fragilis had a higher abundance in the tumor samples than in healthy tissues, and this bacterium may lead to CRC by making changes in cellular signaling pathways and genes. Therefore, to better understand the physiological effects of B. fragilis on the inflammatory response and CRC, future research should focus on dissecting the molecular mechanisms by which this bacterium regulates cellular signaling pathways.

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伊朗结直肠癌患者肠产毒素脆弱拟杆菌与细胞信号通路基因表达的相关性评估

背景:结直肠癌(Colorectal cancer, CRC)是世界范围内最常见的癌症之一，肠道菌群失调可能在结直肠癌的发生中发挥作用。脆弱拟杆菌可通过改变信号通路导致肿瘤发生，包括WNT/β-catenin通路。因此，在本研究中，我们研究了产肠毒素易碎芽孢杆菌数量与CRC相关信号通路基因表达的关系。材料与方法:采用qPCR法对30例肿瘤及邻近健康组织中脆弱芽孢杆菌进行检测。接下来，我们研究了产肠毒素易碎芽孢杆菌与信号通路基因CCND1、TP53、BCL2、BAX、WNT、TCF、AXIN、APC和CTNNB1表达的关系。此外，我们还分析了肿瘤样本的临床病理特征与脆弱芽孢杆菌丰度之间可能存在的相关性。结果:100%的肿瘤组织和86%的健康组织中检出脆弱芽孢杆菌。此外，产肠毒素的脆弱杆菌在所有样本中定植了47%，其中bft-1毒素是样本中最常见的同型。分析表明，脆性芽孢杆菌的高水平与AXIN、CTNNB1和BCL2基因的高表达有显著关系。另一方面，我们的结果没有显示这种细菌与肿瘤样本的临床病理特征之间的任何可能的相关性。结论:脆弱芽孢杆菌在肿瘤样本中的丰度高于健康组织，该细菌可能通过改变细胞信号通路和基因导致结直肠癌的发生。因此，为了更好地了解脆弱芽孢杆菌对炎症反应和结直肠癌的生理作用，未来的研究应重点剖析该细菌调节细胞信号通路的分子机制。

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来源期刊

Infectious Agents and Cancer ONCOLOGY-IMMUNOLOGY

CiteScore

5.80

自引率

2.70%

发文量

期刊介绍： Infectious Agents and Cancer is an open access, peer-reviewed online journal that encompasses all aspects of basic, clinical, epidemiological and translational research providing an insight into the association between chronic infections and cancer. The journal welcomes submissions in the pathogen-related cancer areas and other related topics, in particular: • HPV and anogenital cancers, as well as head and neck cancers; • EBV and Burkitt lymphoma; • HCV/HBV and hepatocellular carcinoma as well as lymphoproliferative diseases; • HHV8 and Kaposi sarcoma; • HTLV and leukemia; • Cancers in Low- and Middle-income countries. The link between infection and cancer has become well established over the past 50 years, and infection-associated cancer contribute up to 16% of cancers in developed countries and 33% in less developed countries. Preventive vaccines have been developed for only two cancer-causing viruses, highlighting both the opportunity to prevent infection-associated cancers by vaccination and the gaps that remain before vaccines can be developed for other cancer-causing agents. These gaps are due to incomplete understanding of the basic biology, natural history, epidemiology of many of the pathogens that cause cancer, the mechanisms they exploit to cause cancer, and how to interrupt progression to cancer in human populations. Early diagnosis or identification of lesions at high risk of progression represent the current most critical research area of the field supported by recent advances in genomics and proteomics technologies.