Hybrid molecularly imprinted polymers for targeted separation and enrichment of 10-hydroxycamptothecin in Camptotheca acuminata Decne

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2024-09-16 DOI:10.1080/14786419.2023.2228981

引用次数: 0

Abstract

The molecularly imprinted polymer was synthesized using 3-aminopropylthiosilane-methacrylic acid monomer (APTES-MAA) as the functional monomer and 10-hydroxycamptothecin (HCPT) as the template, based on computer simulation. The hybrid molecularly imprinted polymers (HMIPs) were characterized using Fourier transform infrared spectroscopy, thermogravimetric analysis, particle size measurement, scanning electron microscopy and energy dispersive X-ray spectroscopy. It has been shown that HMIPs are irregularly shaped and porous, with particle sizes ranging mainly from 130 to 211 nm. At 298 K, the HMIPs exhibit a maximum adsorption capacity of 8.35 mg·g⁻¹ for HCPT and demonstrate good adsorption specificity (α = 5.38). The pseudo-second-order reaction mechanism suggests that the equilibrium adsorption capacity of HCPT on HMIPs is 8.11 mg·g⁻¹. Finally, HCPT was successfully separated and enriched from the extract of Camptotheca acuminata Decne. seeds using HMIPs.

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杂化分子印迹聚合物用于定向分离和富集喜树中的 10-羟基喜树碱。

在计算机模拟的基础上，以 3-aminopropylthiosilane-methacrylic acid monomer (APTES-MAA) 为功能单体，以 10-hydroxycamptothecin (HCPT) 为模板合成了分子印迹聚合物。利用傅里叶变换红外光谱、热重分析、粒度测量、扫描电子显微镜和能量色散 X 射线光谱对混合分子印迹聚合物（HMIPs）进行了表征。研究表明，HMIPs 形状不规则且多孔，粒径主要在 130 至 211 nm 之间。在 298 K 时，HMIPs 对 HCPT 的最大吸附容量为 8.35 mg-g-1，并表现出良好的吸附专一性（α = 5.38）。伪二阶反应机理表明，HMIPs 上 HCPT 的平衡吸附容量为 8.11 mg-g-1。最后，利用 HMIPs 成功地从喜树种子提取物中分离和富集了 HCPT。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.