Proteomics analysis to investigate the potential mechanism of theacrine against UV-induced skin photodamage.

IF 2.5 4区医学 Q2 DERMATOLOGY

Photodermatology, photoimmunology & photomedicine Pub Date : 2023-11-01 Epub Date: 2023-08-29 DOI:10.1111/phpp.12908

Yong He, Xinkai Zheng, Yunfeng Hu, Liehua Deng, Jin Xu, Shi Wu

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引用次数: 0

Abstract

Aim: This study aimed to explore the underlying mechanism of theacrine treatment of UV-induced skin photodamage.

Materials and methods: Tandem Mass Tag (TMT) relative quantitative proteomics analysis was used to characterize the proteins and pathways associated with the ability of theacrine to combat photodamage in mouse skin by modeling UV irradiation of the backs of ICR mice.

Results: Apoptosis-related proteins and signaling pathways play a key role in the ability of theacrine to protect against skin photodamage, according to proteomic and bioinformatics analysis; molecular docking and Western blotting further revealed that theacrine was associated with apoptosis-related proteins (p53, Bcl-2, Bax, caspase-3, and cleaved-caspase-3) with strong binding affinity, which can significantly reduce skin cell apoptosis induced by UV exposure.

Conclusion: The findings revealed that theacrine can reduce UVB-induced epidermal damage by controlling the apoptosis signaling pathway, implying that theacrine could be a useful anti-UVB damage agent.

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蛋白质组学分析探讨抗紫外线皮肤光损伤的潜在机制。

目的:探讨紫外光致皮肤光损伤的机理。材料和方法:采用串联质量标签(TMT)相对定量蛋白质组学分析，通过模拟ICR小鼠背部的紫外线照射，表征与茶树酸抗小鼠皮肤光损伤能力相关的蛋白质和途径。结果:根据蛋白质组学和生物信息学分析，凋亡相关蛋白和信号通路在分泌抗皮肤光损伤的能力中起关键作用;分子对接和Western blotting进一步发现，theacrine与凋亡相关蛋白(p53、Bcl-2、Bax、caspase-3、cleaved-caspase-3)具有较强的结合亲和力，可显著减少紫外线照射引起的皮肤细胞凋亡。结论:茶树碱可通过调控细胞凋亡信号通路减轻uvb诱导的表皮损伤，提示茶树碱可能是一种有效的抗uvb损伤剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photodermatology, photoimmunology & photomedicine 医学-皮肤病学

CiteScore

4.40

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal is a forum for new information about the direct and distant effects of electromagnetic radiation (ultraviolet, visible and infrared) mediated through skin. The divisions of the editorial board reflect areas of specific interest: aging, carcinogenesis, immunology, instrumentation and optics, lasers, photodynamic therapy, photosensitivity, pigmentation and therapy. Photodermatology, Photoimmunology & Photomedicine includes original articles, reviews, communications and editorials. Original articles may include the investigation of experimental or pathological processes in humans or animals in vivo or the investigation of radiation effects in cells or tissues in vitro. Methodology need have no limitation; rather, it should be appropriate to the question addressed.