Effects of MRI on stemness properties of Wharton's jelly-derived mesenchymal stem cells.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-09-01 DOI:10.1007/s10561-022-10052-2

Mahnaz Tashakori, Fatemeh Asadi, Faezeh-Sadat Khorram, Azita Manshoori, Ali Hosseini-Chegeni, Fatemeh Mohseni Moghadam, Mahdieh Ahmadi Kamalabadi, Aliakbar Yousefi-Ahmadipour

{"title":"Effects of MRI on stemness properties of Wharton's jelly-derived mesenchymal stem cells.","authors":"Mahnaz Tashakori, Fatemeh Asadi, Faezeh-Sadat Khorram, Azita Manshoori, Ali Hosseini-Chegeni, Fatemeh Mohseni Moghadam, Mahdieh Ahmadi Kamalabadi, Aliakbar Yousefi-Ahmadipour","doi":"10.1007/s10561-022-10052-2","DOIUrl":null,"url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs), derived from various tissues, are served as a promising source of cells in clinic and regenerative medicine. Umbilical cord-Wharton's jelly (WJ-MSCs)-derived MSCs exhibit advantages over those from adult tissues, such as no ethical concerns, shorter population doubling time, broad differentiation potential, readily available non-invasive source, prolonged maintenance of stemness properties. The aim of this study was to evaluate the effect of MRI (1.5 T, 10 min) on stemness gene expression patterns (OCT-4, SOX-2, NANOG) of WJ-MSCs. Additionally, we assessed cell viability, growth kinetics and apoptosis of WJ-MSCs after MRI treatment. The quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) data showed that transcript levels of SOX-2, NANOG in MRI-treated WJ-MSCs were increased 32- and 213-fold, respectively. MTT assay was performed at 24, 48, and 72 h post-treatment and the viability was not significantly different between the two groups. The doubling time of the MRI group was markedly higher than the control group. In addition, the colony formation ability of WJ-MSCs after MRI treatment significantly increased. Furthermore, no change in apoptosis was seen before or after MRI treatment. Our results suggest that the use of MRI can improve the quality of MSCs and enhance the efficacy of mesenchymal stem cell-based therapies.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s10561-022-10052-2","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Mesenchymal stem cells (MSCs), derived from various tissues, are served as a promising source of cells in clinic and regenerative medicine. Umbilical cord-Wharton's jelly (WJ-MSCs)-derived MSCs exhibit advantages over those from adult tissues, such as no ethical concerns, shorter population doubling time, broad differentiation potential, readily available non-invasive source, prolonged maintenance of stemness properties. The aim of this study was to evaluate the effect of MRI (1.5 T, 10 min) on stemness gene expression patterns (OCT-4, SOX-2, NANOG) of WJ-MSCs. Additionally, we assessed cell viability, growth kinetics and apoptosis of WJ-MSCs after MRI treatment. The quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) data showed that transcript levels of SOX-2, NANOG in MRI-treated WJ-MSCs were increased 32- and 213-fold, respectively. MTT assay was performed at 24, 48, and 72 h post-treatment and the viability was not significantly different between the two groups. The doubling time of the MRI group was markedly higher than the control group. In addition, the colony formation ability of WJ-MSCs after MRI treatment significantly increased. Furthermore, no change in apoptosis was seen before or after MRI treatment. Our results suggest that the use of MRI can improve the quality of MSCs and enhance the efficacy of mesenchymal stem cell-based therapies.

Abstract Image

查看原文本刊更多论文

MRI对Wharton氏胶状间充质干细胞干性的影响。

间充质干细胞(Mesenchymal stem cells, MSCs)来源于多种组织，是临床和再生医学中很有前途的细胞来源。脐带-华顿氏果冻(WJ-MSCs)衍生的MSCs比那些来自成人组织的MSCs具有优势，例如没有伦理问题，群体倍增时间短，分化潜力大，易于获得非侵入性来源，长时间维持干性特性。本研究的目的是评估MRI (1.5 T, 10 min)对WJ-MSCs干性基因表达模式(OCT-4, SOX-2, NANOG)的影响。此外，我们评估了MRI处理后WJ-MSCs的细胞活力、生长动力学和凋亡。定量实时逆转录聚合酶链反应(qRT-PCR)数据显示，在mri处理的WJ-MSCs中，SOX-2、NANOG的转录水平分别增加了32倍和213倍。分别于处理后24、48、72 h进行MTT测定，两组细胞活力无显著差异。MRI组的翻倍时间明显高于对照组。此外，MRI处理后WJ-MSCs的集落形成能力显著增强。此外，在MRI治疗前后，细胞凋亡未见变化。我们的研究结果表明，使用MRI可以改善间充质干细胞的质量，增强间充质干细胞治疗的疗效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.