Multiple Stool Sampling and Specific Parasitological Technique are Crucial to Diagnose Strongyloidiasis in Alcoholic Patients

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2023-08-02 DOI:10.1007/s11686-023-00700-2

Alana A. S. Gonçalves, Camila A. Lopes, Marcelo A. Levenhagen, Henrique T. Gonzaga, Luiz Carlos M. Oliveira, Julia Maria Costa-Cruz

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引用次数: 0

Abstract

Purpose

Chronic alcoholism is a well-known risk factor for strongyloidiasis, in these patients the disease is potentially more severe, probably due to the breakdown of local protective barriers and immunosuppression caused by alcohol, which can lead to autoinfection and dissemination. The aim of this study was to evaluate multiple stool sampling and a specific parasitological assay agar plate culture (APC) for the diagnosis of Strongyloides stercoralis in alcoholics.

Methods

APC was compared to sedimentation technique (HPJ; Hoffman, Pons and Janer), as parasitological methods to detect S. stercoralis infection in alcoholic individuals. Three stool samples from 60 alcoholic and 60 non-alcoholic individuals were analyzed.

Results

S. stercoralis larvae were observed in 11 (18.3%) alcoholic individuals and 1 (1.7%) nonalcoholic individual (P = 0.0042). In view of the combined results, sensitivity for the APC method was 63.6% (CI 31.6–87.6%) with the first sample reaching 100% (CI 67.8–100%) after analyzing three fecal samples. The HPJ sensitivity was 36.4% (CI 12.4–68.4) in the first sample, reaching 72.7% (CI 39.3–92.7) after three samples analyzed.

Conclusion

The present results suggest that in alcoholic patients, it is important to repeat stool sampling with specific techniques, especially using the APC method, to avoid misdiagnosis in cases that could evolve to disseminated strongyloidiasis.

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多份粪便取样和特定的寄生虫学技术对诊断酒精中毒患者的类圆线虫病至关重要

目的慢性酒精中毒是常见的强直性脊柱炎的危险因素，在这些患者中，这种疾病可能更严重，可能是由于酒精引起的局部保护屏障的破坏和免疫抑制，从而导致自身感染和传播。本研究的目的是评估多次粪便取样和特定的寄生虫学分析琼脂平板培养（APC）对酗酒者粪圆线虫病的诊断。方法将APC和沉淀法（HPJ；Hoffman，Pons和Janer）作为检测酒精中毒者粪珊瑚虫感染的寄生虫学方法进行比较。分析了来自60名酗酒者和60名非酗酒者的三份粪便样本。结果。在11个（18.3%）酒精性个体和1个（1.7%）非酒精性个体中观察到珊瑚虫幼虫（P = 0.0042）。鉴于综合结果，APC方法的灵敏度为63.6%（CI 31.6–87.6%），在分析三个粪便样本后，第一个样本达到100%（CI 67.8–100%）。第一个样本的HPJ敏感性为36.4%（CI 12.4–68.4），分析三个样本后达到72.7%（CI 39.3–92.7）。结论目前的结果表明，在酒精中毒患者中，重要的是使用特定的技术重复粪便采样，特别是使用APC方法，以避免可能演变为播散性强线虫病的病例误诊。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.