Comparison of biofilm production and virulence genes distribution among human and canine isolates of Staphylococcus aureus.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2023-01-01 DOI:10.22099/IJVR.2022.43373.6331

A Šmitran, Ž Sladojević, L Božić, I Gajić, T Marković, D Kasagić, I Subić, G Katalina, B Golić

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Abstract

Background: Staphylococcus aureus is an important human and animal pathogen that can cause a wide range of infections due to numerous virulence factors.

Aims: The aim of this study was to compare biofilm formation ability with different virulence factors such as bacterial motility, genes encoding biofilm associated proteins, and Panton-Valentine leukocidin (PVL) among human and canine isolates of S. aureus.

Methods: A total of 60 human (30 methicillin sensitive S. aureus (MSSA) and 30 methicillin resistant S. aureus (MRSA)) and 17 canine (all MSSA) isolates of S. aureus were tested for the capability of biofilm production, motility assay, and presence of genes encoding virulence factors: ica (encoding intercellular adhesion), bap (encoding biofilm-associated protein), fnbA (encoding fibronectin-binding protein A), cna (encoding collagen-binding protein), and pvl (encoding PVL).

Results: Animal isolates of S. aureus performed better biofilm production than the human strains (P=0.042), as well as human MSSA compared to the MRSA isolates (P=0.013). Our results showed that cna, fnbA, and ica genes (67.5%, 66.2%, and 42.9%, respectively) were more prevalent than bap and pvl genes (0%, and 7.8%, respectively). The ica gene was significantly more prevalent in human isolates compared to animal isolates (n=31/60 vs. n=2/17, P=0.008), whereas the cna gene was more frequent in animal isolates than in human ones (n=15/17 vs. n=37/60, P=0.0201). Significant correlations were found between the biofilm formation of animal isolates, and the presence of fnbA (P=0.029) and ica genes (P=0.001).

Conclusion: This study showed a correlation between biofilm production and the presence of certain biofilm-related genes in animal isolates, as well as stronger biofilm production among MSSA human and animal isolates.

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金黄色葡萄球菌人、犬分离株生物膜生成及毒力基因分布的比较。

背景:金黄色葡萄球菌是一种重要的人类和动物病原体，由于许多毒力因素，可引起广泛的感染。目的:本研究的目的是比较人类和犬金黄色葡萄球菌分离株的生物膜形成能力与不同的毒力因素，如细菌活力、生物膜相关蛋白编码基因和潘通-瓦伦丁白细胞杀死素(PVL)。方法:对60株人(30株对甲氧西林敏感的金黄色葡萄球菌(MSSA)和30株耐甲氧西林的金黄色葡萄球菌(MRSA)和17株犬(全部为耐甲氧西林金黄色葡萄球菌)分离的金黄色葡萄球菌进行生物膜生成能力检测、活性测定和毒力因子编码基因的存在:ica(编码细胞间粘附)、bap(编码生物膜相关蛋白)、fnbA(编码纤维连接蛋白结合蛋白A)、cna(编码胶原结合蛋白)和pvl(编码pvl)。结果:金黄色葡萄球菌动物分离株的生物膜产率优于人菌株(P=0.042)，人MSSA产率优于MRSA分离株(P=0.013)。结果表明，cna、fnbA和ica基因(分别为67.5%、66.2%和42.9%)比bap和pvl基因(分别为0%和7.8%)更普遍。ica基因在人分离株中显著高于动物分离株(n=31/60 vs. n=2/17, P=0.008)， cna基因在动物分离株中显著高于人分离株(n=15/17 vs. n=37/60, P=0.0201)。动物分离株的生物膜形成与fnbA基因(P=0.029)和ica基因(P=0.001)存在显著相关。结论:动物分离株的生物膜生成与某些生物膜相关基因的存在存在相关性，并且MSSA人、动物分离株的生物膜生成能力更强。

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来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.