Inhibition of caspase-1-dependent apoptosis suppresses peste des petits ruminants virus replication.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Journal of Veterinary Science Pub Date : 2023-09-01 Epub Date: 2023-07-07 DOI:10.4142/jvs.22288
Lingxia Li, Shengqing Li, Shengyi Han, Pengfei Li, Guoyu Du, Jinyan Wu, Xiaoan Cao, Youjun Shang
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引用次数: 0

Abstract

Background: Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells.

Objectives: PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response.

Methods: Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4+ and CD8+ T cells after PPRV infection.

Results: PPRV stimulated the differentiation of CD4+ and CD8+ T cells. In addition, PPRV induced apoptosis in goat PBMCs. Furthermore, apoptosis and the inflammatory response induced by PPRV could be suppressed by Z-VAD-FMK and Z-YVAD-FMK, respectively. Moreover, the virus titer of PPRV was attenuated by inhibiting caspase-1-dependent apoptosis and inflammation.

Conclusions: This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication in vitro, providing a new mechanism related to the cell host response.

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胱天蛋白酶-1依赖性细胞凋亡的抑制抑制小反刍动物病毒的复制。
背景:小反刍动物害虫(PPR)是由PPR病毒(PPRV)引起的一种急性、致命的传染病,主要感染山羊、绵羊和其他偶蹄目动物。外周血单核细胞(PBMC)被认为是主要的先天免疫细胞。目的:用PPRV感染山羊PBMC,分析PPRV复制与细胞凋亡或炎症反应之间的关系。方法:采用实时定量聚合酶链反应检测PPRV的复制和细胞因子的表达。流式细胞术检测PPRV感染后CD4+和CD8+T细胞的凋亡和分化。结果:PPRV刺激CD4+和CD8+T细胞分化。此外,PPRV诱导山羊PBMC细胞凋亡。此外,Z-VAD-FMK和Z-YVAD-FMK可分别抑制PPRV诱导的细胞凋亡和炎症反应。此外,PPRV的病毒滴度通过抑制胱天蛋白酶1依赖性细胞凋亡和炎症而减弱。结论:细胞凋亡和炎症反应在PPR病毒体外复制中起着重要作用,为细胞-宿主反应提供了一种新的机制。
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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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