Isolation and genome sequencing of a novel lytic Pseudoalteromonas phage SL20

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-10-01 DOI:10.1016/j.margen.2023.101048

Huifang Li , Jie Gao , Shiyun Ma , Rongda Xiao , Xing Zhou , Wanting Feng , Siyu Zhao , Jiaqi Luo , Di Zhang

引用次数: 1

Abstract

Phage SL20, a novel lytic Pseudoalteromonas phage, was isolated from the coastal waters of the Yellow Sea, China. The microbiological characterization demonstrated that phage SL20 was relatively stable from 35 to 55 °C and the optimal pH was approximately 6.0. A latent period of approximately 24 min was indicated by a one-step growth curve. The burst size was approximately 12 ± 3 PFU/cell. The genome had a length of 120,295 bp with a G + C content of 35.84%, and predicted 95 ORFs. The phylogenetic tree based on DNA helicase showed that Pseudoalteromonas phage SL20 was related to the Pseudoalteromonas phage H101 and was a member of the family Shandongvirus. The isolation and genomic analysis of SL20 has improved our understanding of host-phage interactions and the ecology of the marine bacteria Pseudoalteromonas.

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新型假互生单胞菌噬菌体SL20的分离及基因组测序

噬菌体SL20是从黄海近海分离到的一种新型裂解性假互交单胞菌噬菌体。微生物学表征表明，噬菌体SL20在35 ~ 55℃范围内相对稳定，最适pH约为6.0。一步生长曲线显示潜伏期约为24 min。破裂大小约为12±3 PFU/细胞。该基因组全长120295 bp, G + C含量为35.84%，预测orf为95个。基于DNA解旋酶的系统进化树分析表明，假异交单胞菌噬菌体SL20与假异交单胞菌噬菌体H101亲缘关系密切，属于山东病毒科成员。SL20的分离和基因组分析提高了我们对海洋细菌假互生单胞菌宿主-噬菌体相互作用和生态学的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.