Neuroprotective effects of magnesium against stress induced by hydrogen peroxide in Wistar rat.

IF 2 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biomarkers Pub Date : 2023-09-01 Epub Date: 2023-08-27 DOI:10.1080/1354750X.2023.2246104

Latifa Hajri, Haifa Othman, Soumaya Ghodbane, Sakly Mohsen, Hafedh Abdelmelek, Khemais Ben Rhouma, Mohamed Ammari

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引用次数: 0

Abstract

Introduction: Oxidative stress has been implicated in the pathogenesis of diverse disease states. The present study was designed to examine the effects of magnesium sulphate (MgSO₄) against hydrogen peroxide (H₂O₂) induced behaviour impairment and oxidative damage in rats.

Material and methods: Eighteen rats were equally divided into three groups. The first group was kept as a control. In the second group, H₂O₂ was given in drinking water at 3% during 5 days. In the third group, rats were subjected to daily administration of H₂O₂ and MgSO₄ (100 mg/kg; b.w) for 5 days. Animals were subjected to behavioural tests (elevated plus maze and open field). At the end of experiment, brains were extracted for oxidative stress biomarkers assessment including levels of malondialdéhyde and hydrogen peroxide and activities of superoxide dismutase and catalase.

Results: Our findings showed that H₂O₂ treated rat exhibited anxiogenic behaviour and the genesis of free radicals in the brain. Magnesium showed amelioration against oxidative stress and significant decrease in anxiety levels.

Discussion and conclusion: Stress is a powerful process that disrupts brain homeostasis by inducing oxidative stress and its appear that magnesium may have potential therapeutic benefits by reducing oxidative stress and inducing anxiolytic effect.

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镁对过氧化氢诱导的Wistar大鼠应激的神经保护作用。

引言：氧化应激与多种疾病状态的发病机制有关。本研究旨在检测硫酸镁（MgSO4）对过氧化氢（H2O2）诱导的大鼠行为障碍和氧化损伤的影响。材料和方法：将18只大鼠随机分为三组。第一组作为对照。在第二组中，在5 天。在第三组中，大鼠每天施用H2O2和MgSO4（100 mg/kg；b.w）用于5 天。对动物进行行为测试（高架加迷宫和开阔场地）。实验结束时，提取大脑进行氧化应激生物标志物评估，包括丙二醛和过氧化氢的水平以及超氧化物歧化酶和过氧化氢酶的活性。结果：我们的研究结果表明，H2O2处理的大鼠表现出焦虑行为和大脑中自由基的产生。镁对氧化应激有改善作用，焦虑水平显著降低。讨论和结论：应激是一个通过诱导氧化应激破坏大脑稳态的强大过程，镁可能通过减少氧化应激和诱导抗焦虑作用而具有潜在的治疗益处。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomarkers 医学-毒理学

CiteScore

5.00

自引率

3.80%

发文量

140

审稿时长

3 months

期刊介绍： The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.