Expression of macrophage/dendritic cell–related molecules in lymph node sinus macrophages

IF 1.9 4区医学 Q4 IMMUNOLOGY

Microbiology and Immunology Pub Date : 2023-08-25 DOI:10.1111/1348-0421.13095

Rin Yamada, Koji Ohnishi, Cheng Pan, Hiromu Yano, Yukio Fujiwara, Takuya Shiota, Yoshiki Mikami, Yoshihiro Komohara

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Abstract

The role of sinus macrophages (SMs) in anticancer immune responses has received considerable interest in recent years, but the types of molecules that are expressed in human SMs have not yet been clarified in detail. We therefore sought to identify dendritic cell (DC)– or macrophage-related molecules in SMs in human lymph nodes (LNs). SMs are strongly positive for Iba-1, CD163, CD169, and CD209. CD169 (clone SP216) reacted with almost all SMs, mainly in the cell surface membrane, while CD169 (clone HSn 7D2) reacted with a subpopulation of SMs, mainly in the cytoplasm, with a significant increase observed after IFN-α stimulation. The immunoreactivity of clone HSn 7D2 was markedly reduced after transfection with small interfering RNA against CD169, while that of clone SP216 was slightly reduced. The induction of CCL8 and CXCL10 messenger RNA (mRNA) expression by IFN-α was confirmed using cultured macrophages and RT-qPCR, but fluorescence in situ hybridization did not detect CCL8 and CXCL10 mRNA expression in SMs. Single-cell RNA sequence data of LNs indicated that the highest level of CXCL10 gene expression occurred in monocytes. In conclusion, we found that CD209, also known as DC-related molecule, was expressed in human SMs. The heterogeneity observed in CD169 reacted with cone HSn 7D2 and SP216 was potentially due to the modification of CD169 protein by IFN stimulation. Further, no expression of CXCL10 mRNA in SMs suggested that SMs might be resident macrophages.

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淋巴结窦巨噬细胞中巨噬细胞/树突状细胞相关分子的表达。

近年来，窦性巨噬细胞（SM）在抗癌免疫反应中的作用引起了人们的极大兴趣，但在人类SM中表达的分子类型尚未详细阐明。因此，我们试图在人类淋巴结（LNs）的SM中鉴定树突状细胞（DC）或巨噬细胞相关分子。SM对Iba-1、CD163、CD169和CD209呈强阳性。CD169（克隆SP216）与几乎所有的SM反应，主要在细胞表面膜中，而CD169（无性系HSn 7D2）与主要在细胞质中的SM亚群反应，在IFN-α刺激后观察到显著增加。用CD169小干扰RNA转染后，克隆HSn 7D2的免疫反应性显著降低，而克隆SP216的免疫反应活性略有降低。使用培养的巨噬细胞和RT-qPCR证实了IFN-α诱导CCL8和CXCL10信使RNA（mRNA）表达，但荧光原位杂交没有检测到SMs中CCL8或CXCL10 mRNA的表达。LNs的单细胞RNA序列数据表明，CXCL10基因表达最高水平出现在单核细胞中。总之，我们发现CD209，也被称为DC相关分子，在人SM中表达。在CD169与锥体HSn 7D2和SP216反应中观察到的异质性可能是由于IFN刺激对CD169蛋白的修饰。此外，SMs中CXCL10 mRNA的表达不表明SMs可能是常驻巨噬细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.