Are Bacillus thuringiensis strains like any other Bacillus cereus strains? Phenotypic-based tools to locate Bacillus thuringiensis in the diversity of the Bacillus cereus sensu lato group

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2023-07-01 DOI:10.1016/j.resmic.2023.104077

C. Trunet , A. Cauquil , N. Hymery , L. Koullen , F. Postollec , L. Coroller

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引用次数: 1

Abstract

Some Bacillus thuringiensis (Bt) strains are used as pesticide agent. This species belongs to Bacillus cereus (Bc) group which contains many species with a high phenotypic diversity, and could be pathogenic like B. cereus. The aim of this study was to characterize the phenotype of 90 strains belonging to Bc group, half of which were Bt. Knowing that Bt strains belong to different phylogenetic Bc groups, do Bt strains have the same phenotype than other Bc group strains?

Five phenotypic parameters were estimated for 90 strains in the Bc group, of which 43 were Bt strains: minimal, maximal and optimal growth temperature, cytotoxicity on Caco-2 cells, heat resistance of spores. The dataset was processed by principal component analysis, showing that 53% of the variance of the profiles corresponded to factors linked to growth, heat resistance and cytotoxicity. The phenotype followed the phylogenetic groups based on panC. Bt strains showed similar behavior to other strains in the Bc group, in our experimental conditions. Commercial bio-insecticide strains were mesophilic with low heat resistance.

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苏云金芽孢杆菌菌株和其他蜡样芽孢杆菌菌株一样吗?基于表型的工具定位苏云金芽孢杆菌在蜡样芽孢杆菌群的多样性

一些苏云金芽孢杆菌（Bt）菌株被用作杀虫剂。该种属于蜡样芽孢杆菌（Bacillus cereus，Bc）群，该群包含许多具有高度表型多样性的物种，并且可能像蜡状芽孢杆菌一样具有致病性。本研究的目的是对属于Bc群的90株菌株的表型进行表征，其中一半是Bt。已知Bt菌株属于不同的系统发育Bc群，Bt菌株与其他Bc组菌株具有相同的表型吗？对Bc组90株菌株的5个表型参数进行了估计，其中43株为Bt菌株：最低、最高和最佳生长温度、对Caco-2细胞的细胞毒性、孢子的耐热性。该数据集通过主成分分析进行了处理，显示53%的图谱方差与生长、耐热性和细胞毒性相关的因素相对应。表型遵循基于panC的系统发育组。在我们的实验条件下，Bt菌株表现出与Bc组中其他菌株相似的行为。市售生物杀虫剂菌株为中温性菌株，耐热性低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.