Validation of a human paper-based blood typing method for use in pet pigs

IF 1.1 3区农林科学 Q3 VETERINARY SCIENCES

Journal of veterinary emergency and critical care Pub Date : 2023-08-25 DOI:10.1111/vec.13323

Chiara E. Hampton DVM, MS, DACVAA, Xiaojuan Zhu PhD, Luca Giori DVM, PhD

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引用次数: 0

Abstract

Background

Evidence-based medical practices for pet pigs are needed. EldonCard is a human blood-typing card shown to be rapid and reliable in identifying blood phenotypes of pet pigs. The objective of this study was to validate EldonCard by determining its reliability, reproducibility, and robustness for its routine use in the clinical setting.

Key Findings

Twenty-four venous blood samples from pet pigs were collected for a prospective in vitro study. Blood genotypes (“EAA^A0” and “EAA⁰⁰”) were identified in 15 samples via polymerase chain reaction (PCR). All samples were phenotyped (“A,” “A^weak,” and “0” or “–”) via EldonCard. Kappa (κ) statistics measured the level of agreement between 2 raters, and between EldonCard and PCR. McNemar's test determined if an association between the blood types and EldonCard or PCR exists, with significance at P < 0.05. Agreement between raters and methods was perfect (60/60 [100%], κ:1, P < 0.001; 15/15 [100%], κ:1, P < 0.001). There was no difference in the proportions of blood groups based on method.

Significance

In conjunction with previous data, EldonCard is a rapid, accurate, reliable, precise, and robust in-clinic blood-typing method for the A0 system of pet pigs. EldonCard is now a validated blood-typing tool for the A0 system of pet pigs and maybe used for pretransfusion screenings and identification of donors and recipients.

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用于宠物猪的人类纸基血液分型方法的验证。

背景：宠物猪需要循证医学实践。EldonCard是一种人类血型卡，被证明可以快速可靠地识别宠物猪的血液表型。本研究的目的是通过确定EldonCard在临床环境中常规使用的可靠性、再现性和稳健性来验证其有效性。主要发现：从宠物猪身上采集了24份静脉血样本，用于前瞻性体外研究。通过聚合酶链式反应（PCR）在15个样本中鉴定了血液基因型（“EAAA0”和“EAA00”）。所有样本均通过EldonCard进行表型分析（“A”、“Aweak”和“0”或“-”）。Kappa（κ）统计数据测量了2名评分者之间以及EldonCard和PCR之间的一致性水平。McNemar的测试确定了血型与EldonCard或PCR之间是否存在关联，其显著性为P显著性：结合之前的数据，EldonCard是一种快速、准确、可靠、精确且稳健的宠物猪A0系统临床血型分型方法。EldonCard现在是一种经过验证的宠物猪A0系统血型工具，可能用于移植前筛查和捐赠者和接受者的识别。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of veterinary emergency and critical care 农林科学-兽医学

CiteScore

2.50

自引率

15.40%

发文量

121

审稿时长

18-36 weeks

期刊介绍： The Journal of Veterinary Emergency and Critical Care’s primary aim is to advance the international clinical standard of care for emergency/critical care patients of all species. The journal’s content is relevant to specialist and non-specialist veterinarians practicing emergency/critical care medicine. The journal achieves it aims by publishing descriptions of unique presentation or management; retrospective and prospective evaluations of prognosis, novel diagnosis, or therapy; translational basic science studies with clinical relevance; in depth reviews of pertinent topics; topical news and letters; and regular themed issues. The journal is the official publication of the Veterinary Emergency and Critical Care Society, the American College of Veterinary Emergency and Critical Care, the European Veterinary Emergency and Critical Care Society, and the European College of Veterinary Emergency and Critical Care. It is a bimonthly publication with international impact and adheres to currently accepted ethical standards.